Effective, cell permeable and non-toxic spin probe for the detection of superoxide radical and peroxynitrite, both in vitro and in vivo. Resistant to reduction by vitamin C and thiols.
Product Details
| Alternative Name: | 1-Hydroxy-3-carboxy-2,2,5,5-tetramethylpyrrolidine . HCl |
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| Formula: | C9H17NO3 . HCl |
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| MW: | 223.7 |
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| CAS: | 92416-28-9 (non-salt) |
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| Purity: | ≥99% (EPR) |
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| Appearance: | White to off-white solid. |
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| Solubility: | Soluble in DMSO, 100% ethanol, methanol or water. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Prepare fresh solutions daily before use and store 4-6 hours on ice under a nitrogen/argon flow.
Oxygen will lead to oxidation and therefore it is strongly recommended to dissolve the probe in water and not in organic solvents such as DMSO, ethanol o |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Sex differences in blood pro-oxidant status and platelet activation in children admitted with respiratory syncytial virus bronchiolitis: a pilot study: I.T. De Jacobis, et al.; Ital. J. Pediatr.
46, 29 (2020),
Abstract;
Full Text
Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K: H.B. Eajaili, et al.; J. Vis. Exp. e58461 (2019),
Abstract;
An altered redox balance and increased genetic instability characterize primary fibroblasts derived from Xeroderma pigmentosum group A patients: E. Parlanti, et al.; Mutat. Res.
782, 34 (2015),
Application(s): Measurement of ROS levels,
Abstract;
EPR Detection of Cellular and Mitochondrial Superoxide Using Cyclic Hydroxylamines: S.I. Dikalov, et al.; Free. Radic. Res.
45, 417 (2011),
Application(s): Electron paramagnetic resonance (EPR) spectroscopy,
Abstract;
Full Text
Therapeutic targeting of mitochondrial superoxide in hypertension: A.E. Dikalova, et al.; Circ. Res.
107, 106 (2010),
Abstract;
Indoxyl sulfate induces complex redox alterations in mesangial cells: A.K. Gelasco & J.R. Raymond; Am. J. Physiol. Renal Physiol.
290, F1551 (2006),
Abstract;
Interactions of peroxynitrite with uric acid in the presence of ascorbate and thiols: implications for uncoupling endothelial nitric oxide synthase: N. Kuzkaya, et al.; Biochem. Pharmacol.
70, 343 (2005),
Abstract;
Interactions of peroxynitrite, tetrahydrobiopterin, ascorbic acid, and thiols: implications for uncoupling endothelial nitric-oxide synthase: N. Kuzkaya, et al.; J. Biol. Chem.
278, 22546 (2003),
Abstract;
Full Text
Noninvasive diagnostic tool for inflammation-induced oxidative stress using electron spin resonance spectroscopy and an extracellular cyclic hydroxylamine: S.I. Dikalov, et al.; Arch. Biochem. Biophys.
402, 218 (2002),
Abstract;
A new approach for extracellular spin trapping of nitroglycerin-induced superoxide radicals both in vitro and in vivo: B. Fink, et al.; Free Radic. Biol. Med.
28, 121 (2000),
Abstract;
Comparison of glyceryl trinitrate-induced with pentaerythrityl tetranitrate-induced in vivo formation of superoxide radicals: effect of vitamin C: S. Dikalov, et al.; Free Radic. Biol. Med.
27, 170 (1999),
Abstract;
Quantification of superoxide radicals and peroxynitrite in vascular cells using oxidation of sterically hindered hydroxylamines and electron spin resonance: S. Dikalov, et al.; Nitric Oxide
1, 423 (1997),
Abstract;
Spin trapping of superoxide radicals and peroxynitrite by 1-hydroxy-3- carboxy-pyrrolidine and 1-hydroxy-2,2,6, 6-tetramethyl-4-oxo-piperidine and the stability of corresponding nitroxyl radicals towards biological reductants: S. Dikalov, et al.; BBRC
231, 701 (1997),
Abstract;
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