HSP70/HSP72 monoclonal antibody (C92F3A-5) (AP conjugate)



ADI-SPA-810AP-D	50 µg	301.00 USD

ADI-SPA-810AP-F	200 µg	569.00 USD
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Product Details

Alternative Name:	Hsp70, HspA1A, Heat shock protein 70, HspA1B, Hsp72

Clone:	C92F3A-5

Host:	Mouse

Isotype:	IgG1

Immunogen:	Native human Hsp70.

UniProt ID:	P0DMV8 (HSPA1A), P0DMV9 (HSPA1B)

GenBank ID:	M11717

Gene/Protein Identifier:	NP_005336.3 (RefSeq), NM_005345 (RefSeq), 3303 (Entrez GeneID), 140550 (OMIM)

Source:	Purified from ascites.

Species reactivity:	Human, Mouse, Rat Bovine, Chicken, Dog, Fish, Teal

Applications:	ELISA, WB

Recommended Dilutions/Conditions:	Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Purity Detail:	Protein G affinity purified.

Formulation:	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Regulatory Status:	RUO - Research Use Only

HSP70/HSP72 monoclonal antibody (C92F3A-5) (AP conjugate) Western blot

Western blot analysis of HSP70/HSP72, mAb (C92F3A-5) (AP conjugate) (Prod. No. ADI-SPA-810AP): Lane 1: MW marker, Lane 2: HSP70/HSP72 (human), (recombinant) (Prod. No. ADI-NSP-555), Lane 3: HSC70/HSP73 (bovine), (recombinant) (Prod. No. ADI-SPP-751) (Negative Control), Lane 4: HeLa (Prod. No. ADI-LYC-HL100), Lane 5: HeLa (heat shocked) (Prod. No. ADI-LYC-HL101).

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Product Literature References

Possible Adaptation of the Adrenal Gland Hsp72 Expression to Hypoxic Stress: S. Akin, et al.; High Alt. Med. Biol. 22, 293 (2021), Abstract;

Furan fatty acids efficiently rescue brain cells from cell death induced by oxidative stress: A. Teixeira, et al.; Food Funct. 4, 1209 (2013), Abstract;

Augmentation of immune cell activity against tumor cells by Rauwolfia radix: J.C. Cyong, et al. ; J. Ethnopharmacol. 81, 365 (2002), Application(s): EIA using human samples, Abstract;

Estramustine phosphate enhances the effects of hyperthermia and induces the small heat shock protein HSP27 in the human prostate carcinoma cell line PC-3: P.L. Moseley, et al. ; Urol. Res. 30, 130 (2002), Application(s): WB using human samples, Abstract;

Short-term exercise improves myocardial tolerance to in vivo ischemia-reperfusion in the rat: H. Naito, et al. ; J. Appl. Physiol. 91, 2205 (2001), Application(s): WB using rat samples, Abstract;

Stress protein induction in skeletal muscle comparison of laboratory models to naturally occurring hypertrophy: C.R. Ross, et al. ; J. Appl. Physiol. 76, 598 (1994), Application(s): WB using teal samples, Abstract;

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