Product Details
| Alternative Name: | PARK7, Parkinson disease protein 7 |
| |
| Clone: | 3E8 |
| |
| Host: | Mouse |
| |
| Isotype: | IgG1 |
| |
| Immunogen: | Recombinant human DJ-1. |
| |
| UniProt ID: | Q99497 |
| |
| GenBank ID: | BAA09603 |
| |
| Species reactivity: | Human
|
| |
| Applications: | WB
|
| |
| Recommended Dilutions/Conditions: | Western Blot (1-10 µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
| |
| Application Notes: | Detects bands of ~28kDa by Western blot. |
| |
| Purity Detail: | Protein A affinity purified. |
| |
| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | Researchers cloned DJ-1 protein, consisting of 189 amino acids, from a HeLa cell cDNA library as a oncogene which weakly transformed NIH3T3 cells. Cotransfection with ras or c-myc enhances transformation by DJ-11. DJ-1 expression is induced by growth signals, and it is translocated from the cytoplasm to the nucleus during the S phase without the presence of any nuclear localization signals. In addition to its potential involvement in ras-mediated signal transduction pathways, DJ-1 is known to regulate transcription of androgen-responsive genes, and is sensitive to oxidative stress. DJ-1 is ubiquitously expressed in various human tissues including the brain, with high expression in astrocytes of the frontal cortex and substantia nigra. Homozygous mutations in the DJ-1 gene have been shown to cause early-onset autosomal recessive Parkinson’s disease. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Western blot analysis of Jurkat (1), Raji (2), HeLa (3), NIH/3T3 (4) and PC12 (5) using DJ-1 mAb (3E8).
Please mouse over
Product Literature References
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods
15, 909 (2018),
Abstract;
DJ-1 colocalizes with tau inclusions: a link between parkinsonism and dementia: P. Rizzu, et al.; Ann. Neurol.
55, 138 (2004),
Abstract;
Proteomics-based identification of RS/DJ-1 as a novel circulating tumor antigen in breast cancer: S.M. Hanash, et al. ; Clin. Cancer Res.
7, 3328 (2001),
Application(s): WB using human samples,
Abstract;
Related Products
