Product Details
| Alternative Name: | Bcl-2-like protein 3 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to a portion of human Mcl-1. |
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| UniProt ID: | Q07820 |
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| GenBank ID: | L08246 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human
Dog, Guinea pig, Monkey, Porcine, Rabbit
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| Applications: | IF, IHC (PS), WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 1:50)
Western Blot (1:500-1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~42/43kDa by Western blot. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Mcl-1 is a member of the Bcl-2 family that possesses anti-apoptotic activity. Mcl-1 is an immediate-early gene activated by the GMCSF as well as IL-3 signaling pathways and plays an important role in the viability response of these cytokines. Similar to all Bcl-2 family members, Mcl-1 contains BH1, BH2, and BH3 domains; however, unlike other members of this family, Mcl-1 has an extended N-terminal domain rich in PEST sequences, which probably accounts for the short half-life of this protein. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Mcl-1, pAb: Lane 1: MW marker, Lane 2: HeLa, Lane 3: 3T3, Lane 4: PC-12, Lane 5: MDCK, Lane 6: RK-13.
Immunofluorescent analysis (confocal) staining of HeLa cells using Mcl-1 pAb (green); nuclei are stained in blue pseudocolor using DRAQ5.
Immunohistochemistry analysis of human breast cancer tissue with Mcl-1 pAb.
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Product Literature References
A broad-based approach to differentiate CIN from its mimics: The utility of in situ hybridization and immunohistochemistry: G. Nuovo; Ann. Diagn. Pathol.
46, 151515 (2020),
Application(s): IHC on cervical biopsies.,
Abstract;
Combination of fenretinide and ABT-263 induces apoptosis through NOXA for head and neck squamous cell carcinoma treatment: E.L. Britt, et al.; PLoS One
14, e0219398 (2019),
Abstract;
Full Text
Cyclin-dependent kinase-1 (Cdk1)/cyclin B1 dictates cell fate after mitotic arrest via phosphoregulation of antiapoptotic Bcl-2 proteins: N. Sakurinkar, et al.; J. Biol. Chem.
287, 39193 (2018),
Abstract;
Full Text
Increased expression of importin-β, exportin-5 and nuclear transportable proteins in Alzheimer's disease aids anatomic pathologists in its diagnosis: G. Nuovo, et al.; Ann. Diag. Pathol.
32, 10 (2018),
Application(s): Immunohistochemistry on human brain FFPE tissue,
Abstract;
Intracellular Desmoglein-2 cleavage sensitizes epithelial cells to apoptosis in response to pro-inflammatory cytokines: M. Yulis, et al.; Cell Death Dis.
9, 389 (2018),
Abstract;
Full Text
Critical role of mitochondria-mediated apoptosis for JNJ-26481585-induced antitumor activity in rhabdomyosarcoma: U. Heinicke, et al.; Oncogene
35, 3729 (2016),
Application(s): Western Blot,
Abstract;
Low expression of pro-apoptotic Bcl-2 family proteins sets the apoptotic threshold in Waldenström macroglobulinemia: B.T. Gaudette, et al.; Oncogene
35, 479 (2016),
Application(s): Western Blot,
Abstract;
Identification of synthetic lethality of PLK1 inhibition and microtubule-destabilizing drugs: M. Hugle, et al.; Cell Death Differ.
22, 1946 (2015),
Application(s): Western Blot,
Abstract;
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