Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Prediction of Aggregation Propensity and Monitoring of Aggregation of Antibody-Drug Conjugates (ADC) using PROTEOSTAT® Reagents

Download this Application Note

Nicolas Schneider1, Morgan Mathieu2, Sandra Savard-Chambard Gas1, Angélique Boedec Herbette1, Hélène Rispaud1, Naouel Lovera1, Delphine Bregeon1, Agnès Represa1, Christian Belmant1
1 Innate Pharma, Marseille, France
2 Enzo Life Sciences, Lausen, Switzerland

Featured Product: PROTEOSTAT® Protein Aggregation Standards (IgG), PROTEOSTAT® Protein Aggregation Assay, PROTEOSTAT® Thermal Shift Stability Assay


Innate Pharma has developed an antibody-drug conjugate (ADC) coupling technology. The stability of unglycosylated SGN30 Q and S mutants coupled to the MMAE toxin by either one-step (SGN30 Q/S-linker-vcMMAE) or two-step technology (SGN30 Q/S-Linker-Click-Linker-vcMMAE) was compared to the first FDA-approved ADC, ADCETRIS®, and evaluated to validate the technology concept for clinical applications. The temperatures of aggregation, determined via the PROTEOSTAT® Thermal Shift Assay, established the following aggregation propensity prediction ladder: SGN30 Q ADCs coupled with four MMAE toxins are less stable than SGN30 S ADCs coupled with only two MMAE toxins; ADCs coupled with lipophilic linkers are less stable than ADCs coupled with hydrophilic ones; and ADCETRIS® is more stable than a SGN30 Q ADC coupled to a lipophilic linker but less stable than a SGN30 Q ADC coupled with a hydrophilic one. An accelerated degradation study confirmed this hierarchy (with the exception of ADCETRIS® likely due to a higher fragmentation of its antibody element) and provided confirmation that the PROTEOSTAT® Thermal Shift Assay is predictive of aggregation propensity for ADC products.

Read More about Bioprocess


Our bi-weekly TechNotes are an easy way for you to keep in touch with how Enzo Life Sciences is living up to our motto: Scientists Enabling Healthcare™. Each TechNote connects recently published research with one or more of the many solutions Enzo provides for scientists across the life sciences, drug development, and clinical research communities.

Bioprocess TechNotes

Bioprocess Product Range

Better Detection Upstream, Maximum Production Downstream.



Recommend this page