Product Details
Alternative Name: | NADPH dehydrogenase |
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MW: | ~56 kDa |
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Source: | Produced in E. coli. |
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EC: | 1.6.99.1 |
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CAS: | 9001-68-7 |
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Formulation: | Liquid. In 50mM TRIS, pH 8.0, containing 150mM NaCl. |
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Purity: | ≥95% |
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Purity Detail: | Purified by multi-step chromatography. |
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Shipping: | Dry Ice |
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Short Term Storage: | -80°C |
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Long Term Storage: | -80°C |
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Scientific Background: | Diaphorase belongs to a ubiquitous class of flavin-bound enzymes that catalyze the reduction of various dyes which act as hydrogen acceptors from the reduced form of di- and tri- phosphopyridine nucleotides (i.e. NADH, NADPH). |
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Regulatory Status: | RUO - Research Use Only |
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General Literature References
A new assay for diaphorase activity in reagent formulations, based on the reduction of thiazolyl blue: R.S. Boethling & T.L. Weaver; Clin. Chem.
25, 2040 (1979),
Abstract;
The identity of diaphorase and lipoyl dehydrogenase: V. Massey; Biochim. Biophys. Acta.
37, 314 (1960),
Abstract;