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VEGF (human), ELISA kit

Ultra-sensitive ELISA kit for the quantitative detection of human VEGF with 100% cross reactivity to VEGF 165 and negligible cross-reactivity to VEGF 121 and an assay time of just 2.5 hours
ENZ-KIT156-0001 96 wells 585.00 USD
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  • Sensitive Assay - detect VEGF as low as 4.712 pg/ml
  • Specific - 100% cross-reactivity to VEGF 165 with negligible cross-reactivity to related molecules including VEGF 121
  • Flexible - wide dynamic range ideal for various matrices
  • Higher throughput - assay up to 38 samples in duplicate with each kit with an assay time of just 2.5 hours
  • Efficiency - our color coded reagents help to reduce errors and increase efficiency
The VEGF (human), EIA kit is a sensitive colorimetric immunometric enzyme immunoassay kit with results in just 2.5 hours. Absorbance is read at 450 nm. The wide dynamic range is ideal for various matrices. Assay up to 39 samples in duplicate with each kit.
VEGF (human), ELISA kit Standard curve
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VEGF (human), ELISA kit Standard curve

Product Details

Alternative Name:Vascular endothelial growth factor
Sensitivity:4.712 pg/ml (range 8-2,000 pg/ml)
Assay Time:2.5 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of human VEGF in serum, plasma, and culture supernatants.
Wavelength:450 nm
Species reactivity:Human
Crossreactivity:100% cross-reactivity to human VEGF 165. No cross-reactivity to VEGF121, PDGF-AA, IL-10, GRO, Endothelin-1, big Endothelin-1, G-CSF, SCF, IL-8, HGF, Endothelin-3.
Shipping:Blue Ice
Long Term Storage:+4┬░C
Contents:Microtiter Plate, Conjugate, Detector Antibody, Assay buffer, Antibody Diluent, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution
Scientific Background:Vascular Endothelial Growth Factor (VEGF), originally known as vascular permeability factor (VPF), is a family of proteins that stimulate vasculogenesis and angiogenesis. The VEGF family comprises five members: VEGF-A, VEGF-B, VEGF-C, VEGF-D and PGF (placenta growth factor). Being the first VEGF family member discovered, VEGF-A is often simply referred to as VEGF. Alternative splicing of an 8-exon VEGF mRNA results in multiple isoforms of 121, 145, 165, 183, 189, and 206 amino acids. VEGF165 and VEGF121 are predominant isoforms expressed in most cells and tissues. VEGF165 is a 45 KDa homodimeric glycoprotein and has moderate affinity to heparin.  In contrast, VEGF 121 doesn’t bind heparin. VEGF specifically binds tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (Flk-1) to activate downstream signaling pathways leading to endothelial cell survival, proliferation, migration, and vascular permeability. VEGF plays an essential role in normal vascular embryonic and reproductive angiogenesis. On the other hand, VEGF is central to pathological angiogenesis by enhancing vessel permeability or recruiting endothelial progenitor cells to distant sites of neovascularization. The well understanding of VEGF in promoting pathological angiogenesis and tumor metastasis has led to the rational studies on blocking the VEGF/VEGFR axis in inhibiting angiogenesis and tumor growth in multiple clinical models.
Technical Info/Product Notes:Cited samples:
Cytokine ELISAs Cited Samples
UniProt ID:P15692-4
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Negative Modulation of the Angiogenic Cascade Induced by Allosteric Kinesin Eg5 Inhibitors in a Gastric Adenocarcinoma In Vitro Model: A. Ricci, et al.; Molecules 27, 957 (2022), Abstract;
Attenuation of high glucose-induced damage in RPE cells through p38 MAPK signaling pathway inhibition: G. Maugeri, et al.; Front. Pharmacol. 12, 684680 (2021), Application(s): ELISA using culture supernatants, Abstract; Full Text
Mifepristone as a potential therapy to reduce angiogenesis and p-glycoprotein associated with glioblastoma resistance to temozolomide: M. Llaguno-Munive, et al.; Front. Oncol. 10, 581814 (2020), Abstract; Full Text
The combination of sulforaphane and Fernblock® XP improves individual beneficial effects in normal and neoplastic human skin cell lines: S. Serini, et al.; Nutrients 12, 1608 (2020), Application(s): ELISA using culture supernatants, Abstract; Full Text
Aflibercept regulates retinal inflammation elicited by high glucose via the PlGF/ERK pathway: F. Lazzara, et al.; Biochem. Pharmacol. 168, 341 (2019), Application(s): ELISA using culture supernatants, Abstract;
Mechanism of the anti-angiogenic effect of avemar on tumor cells: N.G. Imir, et al.; Oncol. Lett. 15, 2673 (2018), Application(s): ELISA using culture supernatants, Abstract; Full Text
The Role of Human Umbilical Vein Endothelial Cells in Osteogenic Differentiation of Dental Follicle-Derived Stem Cells in In Vitro Co-cultures: J.S. Bok, et al.; Int. J. Med. Sci. 15, 1160 (2018), Abstract; Full Text
The effect of etanercept on vascular endothelial growth factor production by cutaneous mesenchymal stem cells from patients with psoriasis: A. Campanati, et al.; J. Int. Med. Res. 44, 6 (2016), Application(s): VEGF quantification, human MSC cells, Abstract; Full Text

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