Higher sensitivity allows detection of low target concentration
Greater PCR efficiency for accurate and reproducible results
Soluble in water for higher stability
Compatible with various enzyme systems
Suitable for commonly used rotor-type and plate-type qPCR platforms
Validated for qPCR applications and gel staining
Lower cost per assay compared to commonly used dyes
CYGREEN® Nucleic Acid Dye is a DNA intercalating agent that is used to stain DNA. The DNA-dye complex emits a fluorescence spectra that makes it suitable for qPCR and gel staining applications.
CYGREEN® Nucleic Acid Dye has excitation/emission spectra similar to commonly used qPCR dyes and can be substituted in to increase sensitivity and reduce cost.
Product Specification
Concentration:
10,000x
Formulation:
Solution in DMSO.
Excitation maximum:
489nm
Emission maximum:
515nm
Purity:
≥93% (HPLC)
Appearance:
Reddish orange liquid.
Applications:
qPCR, Gel staining, Melting curve
Shipping:
Shipped on Blue Ice
Short Term Storage:
-20°C
Long Term Storage:
-20°C
Handling:
Protect from light. Avoid freeze/thaw cycles.
Regulatory Status:
RUO - Research Use Only
CYGREEN® Nucleic acid dye and 4 other competitor dyes were used in qPCR reactions with varying target concentrations. CYGREEN® Nucleic acid dye has higher sensitivity than competitor dyes and was able to detect low target concentrations.
CYGREEN® Nucleic acid dye and 4 other competitor dyes were used in qPCR reactions with varying target concentrations. CYGREEN® Nucleic acid dye has higher sensitivity than competitor dyes.
DNA Staining before gel electrophoresis. AMPIGENE® DNA Ladder 100-10,000 bp (ENZ-GEN104) was stained with a 1/10 dilution of a 100x solution of CYGREEN® Nucleic Acid Dye (for a 10x final concentration of CYGREEN® Dye) at room temp for 10 min. 15μL of the stained DNA ladder was loaded into each of 3 wells of a 1% agarose gel. Gel was run for 1h at 100V. CYGREEN® Nucleic acid dye prestained DNA can be visualized in a gel.
10μL of CYGREEN® Nucleic Acid Dye was added to 100mL of a 1% Agarose solution. Gel was poured. 15μL of AMPIGENE® DNA Ladder 100-10,000 bp (ENZ-GEN104) was loaded into three lanes of the gel and was run in TAE buffer for 1h at 100v. CYGREEN® Nucleic Acid precast gel showsbright staining.
5, 10 and 15µL of AMPIGENE® DNA Ladder 100-10,000 bp (Prod. No. ENZ-GEN104) were loaded onto a 1% Agarose gel in TAE buffer and run 1h at 100v. Gel was sectioned and stained for 1h with CYGREEN® Dye or with 3 other competitors. CYGREEN® Dye shows bright gel staining equivalent or better than competitors.
Excitation and Emission Spectra of CYGREEN® Nucleic acid dye bound to DNA. CYGREEN® nucleic acid dye shares spectral properties with other commonly used qPCR dyes, allowing detection in the FAM (or similar) channel.
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Product Literature References
A drug-repositioning screen for primary pancreatic ductal adenocarcinoma cells identifies 6-thioguanine as an effective therapeutic agent for TPMT-low cancer cells: I. Kim, et al.; Mol. Oncol. 12, 1526 (2018), Abstract; Full Text
