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Commonly used in molecular biology FRET applications to quench fluorophores
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NHS ester quencher dye can be conjugated to amine-labeled probes
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Enable multiplexing of probes with the use of different reporter-quencher dye pairs
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Compatible with most common commercially available fluorophores
EQ0 NHS ester is an amine-reactive quencher dye. It has a broad visible absorption but no detectable fluorescence emission, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.
It can be used to quench reporter dyes: AMCA, AMCA-X, DEAC, DEAC-X, MCA, MCC, CF™ 350, CF™405S, CF™405M, Pacific Blue™, Cascade Blue®, Marina Blue®, Alexa Fluor® 350, Alexa Fluor® 405, DyLight® 350, DyLight® 405, ATTO 390, ATTO 425, ATTO 465, iFluor™350, iFLuor™405, BD Horizon V450, BUV395, eFluor®450.
Product Details
| MW: | 439.5 |
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| Excitation maximum: | 445 nm |
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| Emission maximum: | none |
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| Extinction Coefficient: | 23,600 M-1cm-1 |
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| Correction Factor (260 nm): | 0.48 |
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| Correction Factor (280 nm): | 0.42 |
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| Purity: | ≥80% (HPLC) |
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| Appearance: | Red brown solid. |
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| Solubility: | Soluble in DMSO or DMF. |
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| Applications: | Fluorescent detection
FRET
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. |
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| Handling: | Protect from moisture. Protect from light. |
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| Scientific Background: | The EQ dyes are a family of non-fluorescent dyes that are designed for fluorescence quenching in FRET applications. |
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| Regulatory Status: | RUO - Research Use Only |
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Normalized absorbance of EQ dyes showing their range of quenching through FRET.
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