| ENZ-ABS362-0100 | 100 µg | 621.00 USD |  |
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| Alternative Name: | GPC3 |
| Clone: | GPC3/1534R |
| Host: | Rabbit |
| Isotype: | IgGκ |
| Immunogen: | A full length human protein was used as the immunogen for the recombinant Glypican-3 antibody. |
| UniProt ID: | P51654 |
| Species reactivity: | Human, Rat |
| Applications: | Flow Cytometry, IF, IHC (PS) |
| Recommended Dilutions/Conditions: | Flow Cytometry (0.5-1 µg/106 cells in 0.1 mL) Immunofluorescence (0.5-1 µg/mL) Immunohistochemistry (formalin-fixed paraffin sections) (0.5-1 µg/mL for 30 min at RT) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Purity Detail: | Protein A affinity purified. |
| Formulation: | Liquid. In 1X PBS containing 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide. |
| Shipping: | Blue Ice |
| Long Term Storage: | +4°C |
| Scientific Background: | Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma. |
| Regulatory Status: | RUO - Research Use Only |
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