Monobromobimane (mBBr) is a thiol-reactive fluorescent probe used for the determination of the redox status of low molecular weight and protein thiols in biological systems. Monobromobimane-based in situ derivatization results in maximal recovery of both free, reduced low molecular weight and monobromobimane-accessible protein thiols. The quantitation of the corresponding adducts of protein thiols is achieved by fluorescence spectroscopy, following protein precipitation. The reagent is particularly useful for quantifying glutathione. Wavelength Maxima: Excitation 395nm, Emission 490nm
Product Details
| Alternative Name: | mBBr, 3-(Bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo(1,2-α) pyrazole-1,7-dione |
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| Formula: | C10H11BrN2O2 |
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| MW: | 271.1 |
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| CAS: | 71418-44-5 |
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| Purity: | ≥95% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Simultaneous and trace determination of reduced and oxidized glutathione in minute plasma samples using dual mode fluorescence detection and column switching high performance liquid chromatography: A.K. Sakhi, et al.; J. Chromatogr. A
1142, 178 (2007),
Abstract;
An improved monobromobimane assay for glutathione utilizing tris- (2-carboxyethyl)phosphine as the reductant: M.T. Anderson, et al.; Anal. Biochem.
272, 107 (1999),
Abstract;
