(6) ROX-12-ddUTP can be used for 3'-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labeled with (6) ROX-12-ddUTP can be detected by direct fluorescence detection (red fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.
Product Details
| Alternative Name: | (6) ROX-12-2’,3’- dideoxyuridine-5’-triphosphate |
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| Formula: | C51H59N6O18P3 |
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| MW: | 1136.96 (free acid) |
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| Concentration: | 0.1mM |
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| Formulation: | Liquid. Solution in water. |
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| Excitation maximum: | 580 nm |
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| Emission maximum: | 603 nm |
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| Extinction Coefficient: | 75,000 M-1 cm-1 (580 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA] |
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| Purity: | ≥93% (HPLC) |
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| Purity Detail: | Purified by ion-exchange chromatography. |
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| Quality Control: | HPLC is used as a quality control to ensure chemical purity of ≥93%. The UV/VIS absorption maximum is obtained in aqueous Tris buffer (10mM Tris, pH 8.0, 1mM EDTA) to determine concentration. |
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| Appearance: | Purple liquid |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Preparation and biomedical applications of programmable and multifunctional DNA nanoflowers: Y. Lv, et al.; Nat. Protoc.
10, 1508 (2015),
Application(s): Labeling of cDNA,
Abstract;
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