Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 


ENZ-42818 250 nmol 315.00 USD
Do you need bulk/larger quantities?
Bio-11-CTP (Biotin-11-cytidine-5’-triphosphate) can replace CTP for in vitro transcription reaction catalyzed by T3, T7 or SP6 RNA polymerases. The biotin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis. The transcription reaction produces multiple RNA copies of the DNA template(s) during a short incubation period. RNA probes offer higher target specificity and greater sensitivity than the corresponding DNA-DNA hybrids. The single-stranded RNA probes offer slectivity unavailable with double-stranded DNA probes, because they are strand-specific. Furthermore, RNA probes hybridize much more efficiently to target molecules than DNA probes because there is no self-hybridization. The biotin-labeled hybridized probes can be detected by a reporter molecule linked to streptavidin, avidin, or anit-biotin antibody. Such a complex can be detected directly, e.g. by excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble colored precipitate.

Product Details

Alternative Name:Biotin-11-cytidine-5’-triphosphate
MW:877.7 (free acid)
Formulation:Liquid. Solution in water.
Extinction Coefficient:9,100 M-1 cm-1 (271 nm, pH 7.0)
Purity:≥93% (HPLC)
Purity Detail:Contains <5% Bio-11-CDP.
Appearance:Clear, colorless liquid.
Shipping:Dry Ice
Long Term Storage:-20°C
Handling:Avoid freeze/thaw cycles.
Technical Info/Product Notes:Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.

Regulatory Status:RUO - Research Use Only
Please mouse over

Product Literature References

Loss of RNA binding protein HuD facilitates the production of the senescence-associated secretory phenotype: S. Ryu, et al.; Cell Death Dis. 13, 329 (2022), Abstract;
RNA binding protein HuD mediates the crosstalk between β cells and islet endothelial cells by the regulation of Endostatin and Serpin E1 expression: M. Jung, et al.; Cell Death Dis. 13, 1019 (2022), Abstract;
Metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes: P. Thongsripong, et al.; Sci. Rep. 11, 8448 (2021), Abstract;
SRSF3 Is a Critical Requirement for Inclusion of Exon 3 of BIS Pre-mRNA: J.Y. Baek, et al.; Cells 9, 2325 (2020), Application(s): Biotin Pull-Down Assay, Abstract; Full Text
Polypyrimidine tract-binding protein 1-mediated down-regulation of ATG10 facilitates metastasis of colorectal cancer cells: Y. K. Jo, et al.; Cancer Lett. 385, 21 (2017), Abstract;
Heterogeneous nuclear ribonucleoprotein A1 post-transcriptionally regulates Drp1 expression in neuroblastoma cells: S.J. Park, et al.; Biochim. Biophys. Acta 1849, 1423 (2015), Application(s): Biotin pull-down assay, Abstract;
Preparation of mRNA for expression monitoring: M.C. Byrne, et al.; Curr. Protoc. Neurosci. Chapter 4, Unit 4.26 (2001), Abstract;

Product Toolbox


Certificate of Analysis


By catalog section:


Technical Service
Customer Service

Related Literature

Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

All new literature pieces