Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .
Product Details
| Alternative Name: | Cyanine 3-uridine-5’-triphosphate (enhanced) |
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| Concentration: | 10mM |
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| Formulation: | Liquid. Solution in water. |
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| Excitation maximum: | 550 nm |
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| Emission maximum: | 564 nm |
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| Extinction Coefficient: | 150,000 M-1 cm-1 (550 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA]) |
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| Purity: | ≥93% (HPLC) |
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| Purity Detail: | Purified by ion-exchange chromatography. |
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| Appearance: | Dark pink liquid. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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41, 111800 (2022),
Abstract;
Borna disease virus phosphoprotein triggers the organization of viral inclusion bodies by liquid-liquid phase separation: Y. Hirai, et al.; Int. J. Biol. Macromol.
192, 55 (2021),
Abstract;
NORAD-induced Pumilio phase separation is required for genome stability: M.M. Elguindy, et al.; Nature
595, 303 (2021),
Abstract;
Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression: H. Kim, et al. ; Sci. Rep.
5, 12737 (2015),
Application(s): Labeling of mRNA-NPs,
Abstract;
