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Muscle actin monoclonal antibody (HHF35) (Ready-to-Use)

ENZ-30931 6 ml ~6 µg 256.00 USD
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Alternative size available: ENZ-C34931 (0.5ml)

Product Details

Immunogen:Human myocardium (proteins extracted in 2% SDS).
UniProt ID:P68133 (ACTA1), P63267 (ACTG2)
Source:Purified from hybridoma tissue culture supernatant.
Species reactivity:Human
Monkey, Rabbit
Specificity:Recognizes actin isotypes α and γ of skeletal, cardiac and smooth muscle. This antibody labels rhabdomyosarcomas, hemangiopericytomas, leiomyosarcomas and leiomyomas but does not react with non-myogenic sarcomas. It is nonreactive with other neoplasms such as carcinoma or lymphoma and with other mesenchymal and all epithelial cells, with the exception of myoepithelium.
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections; this antibody can be used for staining of paraffin-embedded tissue sections fixed in neutral buffered formalin and is compatible with most commonly used histological fixatives. Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Affinity purified.
Formulation:Liquid. Ready-to-use solution in PBS containing 0.1% sodium azide and 1% BSA.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:+4°C
Regulatory Status:RUO - Research Use Only

Product Literature References

Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins: R.A. Schmidt, et al.; Am. J. Pathol. 131, 19 (1988), Abstract; Full Text
Diagnosis of Rhabdomyosarcomas using HHF35, an anti-muscle actin monoclonal antibody: R.A. Schmidt, et al.; Lab. Invest. 56, 70A (1987),
HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues: T. Tsukada, et al.; Am. J. Pathol. 127, 389 (1987), Abstract; Full Text
HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization: T. Tsukada, et al.; Am. J. Pathol. 126, 51 (1987), Abstract; Full Text
Human atherosclerosis. II. Immunocytochemical analysis of the cellular composition of human atherosclerotic lesions: A.M. Gown, et al.; Am. J. Pathol. 125, 191 (1986), Abstract; Full Text
CGA-7 and HHF, two monoclonal antibodies that recognize muscle actin and react with adherent cells in human long-term bone marrow cultures: P. Charbord, et al.; Blood 66, 1138 (1985), Abstract; Full Text

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Muscle actin monoclonal antibody (HHF35) 

Purified from hybridoma tissue culture supernatant., IHC (PS), WB | Print as PDF
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