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High purity industry standard substrate for deubiquitinylating enzymes (DUBs).
BML-SE761-0025 25 µg 230.00 USD
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Ubiquitin-Rhodamine is prepared by the C-terminal derivatization of ubiquitin with modified rhodamine-110 and has been shown to be a useful and sensitive fluorogenic substrate for wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs).  Ubiquitin-rhodamine is particularly useful for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential.  The long excitation and emission wavelengths of rhodamine 110 (Excitation 485nm / Emission 520nm) avoid interference often caused at shorter wavelengths by small molecules and tissue culture components.

Product Details

Sequence:Accession number: P62988; length: 76 amino acid residues.
Source:Produced in E. coli and covalently attached to modified rhodamine-110.
UniProt ID:P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
Formulation:Liquid. In dimethylsulfoxide.
Purity:≥95% (HPLC)
Purity Detail:Please note: considerable efforts are taken to ensure that ubiquitin-rhodamine is homogeneous and not contaminated with ubiquitin and ubiquitin deletions, which are common in material obtained from other sources.  The chromatogram below demonstrates the homogeneity of Prod. No. BML-SE761.
Application Notes:Can be used for the following applications:
1. Substrate for deubiquitinylating enzyme activity assays.
2. Identification/confirmation of enzyme deubiquitinylation activity.
3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL substrates.
Shipping:Dry Ice
Short Term Storage:-80°C
Long Term Storage:-80°C
Use/Stability:Stable for at least one year after receipt when stored at -80°C. After reconstitution, prepare aliquots and store at -80°C.
Handling:Avoid freeze/thaw cycles.
Emission maximum:Excitation 485nm / Emission 520nm
Regulatory Status:RUO - Research Use Only
Ubiquitin-Rhodamine Fig2
HPLC analysis of BML-SE761.
Ubiquitin-Rhodamine Fig1
DUB Assay: 50nM USP2 (Prod. No. BML-UW9850); 500nM Ubiquitin-Rhodamine; 50mM HEPES pH7.8, 0.5mM EDTA, 1mM DTT, and ovalbumin 1mg/ml.
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Ubiquitin-Rhodamine Fig2 Ubiquitin-Rhodamine Fig1

General Literature References

A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine110-glycine as substrate.: U. Hassiepen; Anal. Biochem. 371, 201 (2007), Abstract;

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