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Ubiquitin-Rhodamine

High purity industry standard substrate for deubiquitinylating enzymes (DUBs).



BML-SE761-0025	25 µg	230.00 USD
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Ubiquitin-Rhodamine is prepared by the C-terminal derivatization of ubiquitin with modified rhodamine-110 and has been shown to be a useful and sensitive fluorogenic substrate for wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). Ubiquitin-rhodamine is particularly useful for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential. The long excitation and emission wavelengths of rhodamine 110 (Excitation 485nm / Emission 520nm) avoid interference often caused at shorter wavelengths by small molecules and tissue culture components.

Product Details

Sequence:	Accession number: P62988; length: 76 amino acid residues.

MW:	~8.9kDa

Source:	Produced in E. coli and covalently attached to modified rhodamine-110.

UniProt ID:	P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)

Formulation:	Liquid. In dimethylsulfoxide.

Purity:	≥95% (HPLC)

Purity Detail:	Please note: considerable efforts are taken to ensure that ubiquitin-rhodamine is homogeneous and not contaminated with ubiquitin and ubiquitin deletions, which are common in material obtained from other sources. The chromatogram below demonstrates the homogeneity of Prod. No. BML-SE761.

Application Notes:	Can be used for the following applications: 1. Substrate for deubiquitinylating enzyme activity assays. 2. Identification/confirmation of enzyme deubiquitinylation activity. 3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL substrates.

Shipping:	Dry Ice

Short Term Storage:	-80°C

Long Term Storage:	-80°C

Use/Stability:	Stable for at least one year after receipt when stored at -80°C. After reconstitution, prepare aliquots and store at -80°C.

Handling:	Avoid freeze/thaw cycles.

Emission maximum:	Excitation 485nm / Emission 520nm

Regulatory Status:	RUO - Research Use Only

HPLC analysis of BML-SE761.

DUB Assay: 50nM USP2 (Prod. No. BML-UW9850); 500nM Ubiquitin-Rhodamine; 50mM HEPES pH7.8, 0.5mM EDTA, 1mM DTT, and ovalbumin 1mg/ml.

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General Literature References

A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine110-glycine as substrate.: U. Hassiepen; Anal. Biochem. 371, 201 (2007), Abstract;