| Alternative Name: | Abelson murine leukemia viral oncogene homolog 1 |
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| Sequence: | Mouse recombinant Abl1 (aa 27-1123) with N-terminal histidine tag. Mouse Abl1 is 89% identical (91% similar) to human Abl1. |
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| MW: | ~135kDa |
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| Source: | Produced in insect cells. Produced in a baculovirus expression system. |
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| UniProt ID: | P00520 |
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| Concentration: | 0.1mg/ml |
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| Formulation: | Liquid. In 50mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
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| Purity: | ≥70% (SDS-PAGE) |
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| Specific Activity: | 820 nmol/min/mg using a peptide substrate, EAIYAAPFAKKK, in 25mM MOPS, pH 7.2, containing 12.5mM β-glycerol-phosphate, 25mM MgCl2, 5mM EGTA, 2mM EDTA. Add 0.25mM DTT to assay buffer prior to use. |
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| Application Notes: | Useful tool to study enzyme regulation and kinetics, phosphorylate target substrates, screen for inhibitors. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in cell differentiation, cell division, cell adhesion, and stress responses. The c-Abl protein is inhibited by its SH3 domain, and deletion of this domain turns ABL1 into an oncogene. A chromosomal translocation results in the head-to-tail fusion of the BCR and ABL1 genes. This translocation is present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of Abl1 is regulated by CDC2-mediated phosphorylation. |
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| Regulatory Status: | RUO - Research Use Only |
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