Product Details
| MW: | ~30 kDa |
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| Source: | Produced in E. coli. Human 14-3-3τ/θ is fused at the C-terminus to a His-tag. |
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| UniProt ID: | P27348 |
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| Formulation: | Liquid. In 50mM NaH2PO4, pH 8.0, 300mM NaCl. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Application Notes: | Drug screening, protein-protein interaction studies, gel overlays. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Scientific Background: | 14-3-3 proteins are dimeric phosphoserine/phosphothreonine binding proteins, which have been shown to be involved in the regulation of many crucial processes such as signal transduction, cell-cycle control, apoptosis, protein localization, phosphorylation state and stability, transcription, metabolism, and malignant transformation. 14-3-3θ/t has been shown to be present in testis and 14-3-3θ mRNA is up-regulated in amyotrophic lateral sclerosis spinal cord. Both PKCµ and Bax have been shown to be negatively regulated by 14-3-3θ |
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| Regulatory Status: | RUO - Research Use Only |
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General Literature References
C-TAK1 interacts with microphthalmia-associated transcription factor, Mitf, but not the related family member Tfe3: T. Schwarz, et al.; BBRC
394, 890 (2010),
Abstract;
14-3-3 Interacts directly with and negatively regulates pro-apoptotic Bax.: M. Nomura et al.; J Biol Chem
278, 2058 (2003),
Abstract;
How do 14-3-3 proteins work? Gatekeeper phosphorylation and the molecular anvil hypothesis.: M.B. Yaffe; FEBS Lett.
513, 53 (2002),
Abstract;
A 14-3-3 mRNA is up-regulated in amyotrophic lateral sclerosis spinal cord.: A. Malaspina et al.; J. Neurochem.
75, 2511 (2000),
Abstract;
Characterization of a novel transcript of 14-3-3 theta in Sertoli cells.: J. Chaudhary & M.K. Skinner; J. Androl.
21, 730 (2000),
Abstract;
Protein kinase C mu is negatively regulated by 14-3-3 signal transduction proteins.: A. Hausser et al.; J. Biol. Chem.
274, 9258 (1999),
Abstract;
14-3-3 proteins interact with the insulin-like growth factor receptor but not the insulin receptor.: R.W. Furlanetto et al.; Biochem. J.
327, 765 (1997),
Abstract;
Molecular cloning and tissue-specific expression of the mouse homologue of the rat brain 14-3-3 theta protein: characterization of its cellular and developmental pattern of expression in the male germ line.: L. Perego & G. Berruti; Mol. Reprod. Dev.
47, 370 (1997),
Abstract;
