| Alternative Name: | KDR, FLK-1, Vascular endothelial growth factor receptor 2 |
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| MW: | ~87 kDa |
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| Source: | Produced in Sf9 insect cells with N-terminal GST tage. Human VEGFR2 (aa 805-1356). Produced in a baculovirus expression system. |
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| UniProt ID: | P35968 |
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| GenBank ID: | AF035121 |
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| Formulation: | Liquid. In 40mM TRIS-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 20% Glycerol, and 3mM DTT. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Specific Activity: | 56 U/μg. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of tyrosine substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 μM Naorthovanadate, 0.5 mM ATP, 30 μg/ml Poly (Glu:Tyr)4:1 substrate, and 0.04 ug recombinant VEGFR2. |
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| Application Notes: | Useful tool to study enzyme regulation and kinetics, phosphorylate target substrates, screen for inhibitors. |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | When stored under the above conditions, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing, could lead to loss of activity. |
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| Handling: | After opening, prepare aliquots, freeze in liquid nitrogen and store at -80°C. Avoid freeze/thaw cycles. |
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| Regulatory Status: | RUO - Research Use Only |
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