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[K(Ac)40]α-Tubulin polyclonal antibody

BML-SA452-0100 100 µl 487.00 USD
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Product Details

Immunogen:Synthetic acetylated peptide (K(Ac)40) corresponding to aa 35-45 (Q35MPSD-K(Ac)-TIGGG45) of human α-tubulin.
UniProt ID:Q71U36 (TUBA1A), P68363 (TUBA1B), P0DPH7 (TUBA3C)
Source:Purified from rabbit serum.
Species reactivity:Chicken, Primate, Rodent, Xenopus, Zebrafish
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~50kDa by Western blot.
Formulation:Liquid. In PBS.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
[K(Ac)40]α-Tubulin polyclonal antibody Western blot
Treatment of A549 cells with the histone deacetylase inhibitor trichostatin A (TSA, 0.3 µM, 7 hr.) induces acetylation of alpha-tubulin-Lys40. Western blot analysis was performed with A549 cell extracts (100,000 cells per lane) from either vehicle treated (Control) or TSAtreated (+TSA) cells. All lanes were probed with a 1:1000 dilution of the primary antibody, anti-alpha-tubulin (K(Ac)40) (Prod. No. BML-SA452). For lanes 2 and 5 the primary antibody was preincubated with the immunogen peptide (Ac-QMPSD-K(Ac)-TIGGG[CNH2] and for lanes 3 and 6 it was preincubated with non-acetylated peptide Ac-QMPSDKTIGGG[C-NH2] (peptides: 0.5 µg/µl, antibody: 1:100 dilution, 37°C, 1hr.). Secondary antibody for all lanes was donkey anti-rabbit-AP (1:2000; Jackson Immunoresearch) and the nitrocellulose membrane was developed with BCIP/NBT.
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[K(Ac)40]α-Tubulin polyclonal antibody Western blot

Product Literature References

Differences in Functional Expression of Connexin43 and NaV1.5 by Pan- and Class-Selective Histone Deacetylase Inhibition in Heart: X. Zhang, et al.; Int. J. Mol. Sci. 19, E2288 (2018), Abstract; Full Text
Histone deacetylase inhibition reduces cardiac connexin43 expression and gap junction communication: Q. Xu, et al.; Front. Pharmacol. 4, 44 (2013), Application(s): Western blot, Abstract; Full Text

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