Product Details
| Host: | Rabbit |
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| Immunogen: | Synthetic acetylated peptide (K(Ac)40) corresponding to aa 35-45 (Q35MPSD-K(Ac)-TIGGG45) of human α-tubulin. |
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| UniProt ID: | Q71U36 (TUBA1A), P68363 (TUBA1B), P0DPH7 (TUBA3C) |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Chicken, Primate, Rodent, Xenopus, Zebrafish
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~50kDa by Western blot. |
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| Formulation: | Liquid. In PBS. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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![[K(Ac)40]α-Tubulin polyclonal antibody Western blot](https://www.enzolifesciences.com/fileadmin/files/image/BML-SA452_WB.jpg "[K(Ac)40]α-Tubulin polyclonal antibody Western blot")
Treatment of A549 cells with the histone deacetylase inhibitor trichostatin A (TSA, 0.3 µM, 7 hr.) induces acetylation of alpha-tubulin-Lys40. Western blot analysis was performed with A549 cell extracts (100,000 cells per lane) from either vehicle treated (Control) or TSAtreated (+TSA) cells. All lanes were probed with a 1:1000 dilution of the primary antibody, anti-alpha-tubulin (K(Ac)40) (Prod. No. BML-SA452). For lanes 2 and 5 the primary antibody was preincubated with the immunogen peptide (Ac-QMPSD-K(Ac)-TIGGG[CNH2] and for lanes 3 and 6 it was preincubated with non-acetylated peptide Ac-QMPSDKTIGGG[C-NH2] (peptides: 0.5 µg/µl, antibody: 1:100 dilution, 37°C, 1hr.). Secondary antibody for all lanes was donkey anti-rabbit-AP (1:2000; Jackson Immunoresearch) and the nitrocellulose membrane was developed with BCIP/NBT.
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Product Literature References
Differences in Functional Expression of Connexin43 and NaV1.5 by Pan- and Class-Selective Histone Deacetylase Inhibition in Heart: X. Zhang, et al.; Int. J. Mol. Sci.
19, E2288 (2018),
Abstract;
Full Text
Histone deacetylase inhibition reduces cardiac connexin43 expression and gap junction communication: Q. Xu, et al.; Front. Pharmacol.
4, 44 (2013),
Application(s): Western blot,
Abstract;
Full Text
![[K(Ac)40]α-Tubulin polyclonal antibody Western blot](https://www.enzolifesciences.com/fileadmin/thumbs/dd5ae454969143cb89bc22210ef9e9ea482b2cad-BML-SA452_WB.jpg.jpg "[K(Ac)40]α-Tubulin polyclonal antibody Western blot")
