Product Details
| Clone: | P4D1 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Denatured bovine ubiquitin. |
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| UniProt ID: | P0CG53 (UBB), P0CH28 (UBC), P62992 (RPS27A), P63048 (UBA52) |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Species independent
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| Specificity: | Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and free ubiquitin. |
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| Applications: | IHC, IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Clone P4D1 has been reported to be of utility in immunoprecipitation, immunofluorescence and immunohistochemistry (see Brady et al. (2005); Wang et al. (2008); Ito et al. (2003) respectively). |
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| Purity Detail: | Protein G affinity purified form cell culture media. |
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| Formulation: | Liquid. In PBS containing 0.01% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Ubiquitin modification of cellular proteins has been shown to be involved with a wide range of biochemical processes including proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy. |
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| Technical Info/Product Notes: | The ubiquitin antibody BML-PW0930 (clone P4D1) was developed using a denatured glutaraldehyde cross-linked ubiquitin IgG complex as the immunogen. Monoclonal antibody was purified from tissue culture supernatant by ion exchange chromatography. The general ubiquitin reactivity of the monoclonal antibody has been demonstrated by Western blotting using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in cell lysates. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
14-3-3 proteins regulate cullin 7-mediated Eag1 degradation: C.H. Hsieh, et al.; Cell Biosci.
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hCINAP regulates the differentiation of embryonic stem cells by regulating NEDD4 liquid-liquid phase-separation-mediated YAP1 activation: R. Zhuge, et al.; Cell Rep.
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USP41 Enhances Epithelial–Mesenchymal Transition of Breast Cancer Cells through Snail Stabilization: J.Y. Yoon, et al.; Int. J. Mol. Sci.
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A versatile new tool derived from a bacterial deubiquitylase to detect and purify ubiquitylated substrates and their interacting proteins: M. Zhang, et al.; PLoS Biol.
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Assembly checkpoint of the proteasome regulatory particle is activated by coordinated actions of proteasomal ATPase chaperones: A. Nahar, et al.; Cell Rep.
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Deubiquitinase OTUD6A promotes breast cancer progression by increasing TopBP1 stability and rendering tumor cells resistant to DNA-damaging therapy: Y. Zhao, et al.; Cell Death Differ.
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The RNA helicase DHX16 recognizes specific viral RNA to trigger RIG-I-dependent innate antiviral immunity: A. Hage, et al.; Cell Rep.
38, 110434 (2022),
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Ubiquitination of Ebola virus VP35 at lysine 309 regulates viral transcription and assembly: S. van Tol, et al.; PLoS Pathog.
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Yeast Smy2 and its human homologs GIGYF1 and -2 regulate Cdc48/VCP function during transcription stress: M.H. Lehner, et al.; Cell Rep.
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20S proteasomes secreted by the malaria parasite promote its growth: E. Dekel, et al.; Nat. Commun.
12, 1172 (2021),
Abstract;
Full Text
In-depth proteomic analysis of proteasome inhibitors bortezomib, carfilzomib and MG132 reveals that mortality factor 4-like 1 (MORF4L1) protein ubiquitylation is negatively impacted: T.R. Porras-Yakushi, et al.; J. Proteomics
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Novel role of xanthine oxidase-dependent H2O2 production in 12/15-lipoxygenase-mediated de novo lipogenesis, triglyceride biosynthesis and weight gain: S. Govatati, et al.; Redox Biol.
47, 102163 (2021),
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Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation: M. Ranes, et al.; Mol. Cell
81, 8460 (2021),
Abstract;
Spatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model: M.J. Kumar, et al.; Sci. Rep.
11, 96 (2021),
Abstract;
Full Text
The deubiquitinase TRABID stabilises the K29/K48-specific E3 ubiquitin ligase HECTD1: L. Harris, et al.; J. Biol. Chem.
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The role of TNF-α and IFN-γ in the hyperglycemia-induced ubiquitination and loss of PECAM-1 in rat retinal endothelial cells: R.S. Eshaq, et al.; Microcirculation (2021),
Abstract;
USP18 promotes tumor metastasis in esophageal squamous cell carcinomas via deubiquitinating ZEB1: C. Song, et al.; Exp. Cell Res.
409, 112884 (2021),
Abstract;
Drosophila NUAK functions with Starvin/BAG3 in autophagic protein turnover: D. Brooks, et al.; PLoS Genet.
16, e1008700 (2020),
Abstract;
Full Text
Curcumin improves exercise performance of mice with coronary artery ligation-induced HFrEF: Nrf2 and antioxidant mechanisms in skeletal muscle: A.M. Wafi, et al.; J. Appl. Physiol.
126, 477 (2019),
Abstract;
Disruption of valosin-containing protein activity causes cardiomyopathy and reveals pleiotropic functions in cardiac homeostasis: M.J. Brody, et al.; J. Biol. Chem.
294, 8918 (2019),
Abstract;
Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis: J. Ferreira, et al.; PLoS One
14, e0223790 (2019),
Abstract;
Full Text
Host-Specific NS5 Ubiquitination Determines Yellow Fever Virus Tropism: L. Miorin, et al.; J. Virol.
93, e00151-19 (2019),
Abstract;
DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis: A. Nassrallah, et al.; Elife
7, e37892 (2018),
Abstract;
Full Text
Novel Regulatory Roles of Wnt1 in Infection-Associated Colorectal Cancer: J. Wang, et al.; Neoplasia
20, 499 (2018),
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Full Text
The Monocot-Specific Receptor-like Kinase SDS2 Controls Cell Death and Immunity in Rice: J. Fan, et al; Cell Host Microbe
23, 498 (2018),
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Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology: Y.C. Lee, et al.; J. Biomed. Sci.
25, 76 (2018),
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Full Text
Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease: P. Luningschror, et al.; Nat. Commun.
8, 678 (2017),
Abstract;
Full Text
Ubiquitin Chain Enrichment Middle-Down Mass Spectrometry (UbiChEM-MS) Reveals Cell-Cycle Dependent Formation of Lys11/Lys48 Branched Ubiquitin Chains: A.S.J.B. Rana, et al.; J. Proteome Res.
16, 3363 (2017),
Abstract;
Full Text
Extracellular stimulation of VSIG4/complement receptor Ig suppresses intracellular bacterial infection by inducing autophagy: K.H. Kim, et al.; Autophagy
12, 1647 (2016),
Abstract;
Full Text
FADD regulates NF-κB activation and promotes ubiquitination of cFLIPL to induce apoptosis: K. Ranjan, et al.; Sci. Rep.
6, 22787 (2016),
Application(s): Western blot,
Abstract;
Full Text
NEDD4-mediated HSF1 degradation underlies α-synucleinopathy: E. Kim, et al.; Hum. Mol. Genet.
25, 211 (2016),
Application(s): In vivo ubiquitination assay,
Abstract;
Full Text
Proteasome Activation is Mediated via a Functional Switch of the Rpt6 C-terminal Tail Following Chaperone-dependent Assembly: V. Sokolova, et al.; Sci. Rep.
5, 14909 (2015),
Abstract;
Full Text
UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP: Y. Teng, et al.; Biochem. Pharmacol.
97, 518 (2015),
Application(s): Immunoblotting,
Abstract;
Quality control of oxidatively damaged mitochondrial proteins is mediated by p97 and the proteasome: C. Hemion, et al.; Free Radic. Biol. Med.
75, 121 (2014),
Abstract;
General Literature References
A role for ubiquitin in selective autophagy: V. Kirkin, et al.; Mol. Cell.
34, 259 (2009),
Abstract;
Analysis of nondegradative protein ubiquitylation with a monoclonal antibody specific for lysine-63-linked polyubiquitin: H. Wang, et al.; PNAS
105, 20197 (2008),
Abstract;
Proteasome-independent functions of ubiquitin in endocytosis and signaling: D. Mukhopadhyay & H. Riezman; Science
315, 201 (2007),
Abstract;
Regulation of p53 and MDM2 activity by MTBP: M. Brady, et al.; Mol. Cell Biol.
25, 545 (2005),
Abstract;
Dorfin localizes to Lewy bodies and ubiquitylates synphilin-1: T. Ito, et al.; J. Biol. Chem.
278, 29106 (2003),
Abstract;
TAK1 is a ubiquitin-dependent kinase of MKK and IKK: C. Wang, et al.; Nature
412, 346 (2001),
Abstract;
DOT4 links silencing and cell growth in Saccharomyces cerevisiae: A. Kahana & D.E. Gottschling; Mol. Cell Biol.
19, 6608 (1999),
Abstract;
The ubiquitin-proteasome pathway: on protein death and cell life: A. Ciechanover; EMBO J.
17, 7151 (1998),
Abstract;
