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ADAM substrate (fluorogenic)

BML-P235-0001 1 mg 246.00 USD
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Fluorogenic substrate for ADAM8 (kcat/Km = 1.0 x 105 M-1s-1), ADAM10 (6.2 x 103), ADAM12 (2.8 x 105), TACE (TNF-α converting enzyme, ADAM17) (4.3 x 105), and likely other ADAMs, especially those that cleave TNF-α type sequences. Also efficiently cleaved by MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-13, and MMP-14. FAM fluorescence is quenched by the Dabcyl group until cleavage separates them. Ex.: 485nm, Em.: 530nm. This substrate is useful for inhibitor screening and kinetic analysis.

Product Details

Sequence:Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(5-FAM)-NH2 . TFA (Dabcyl=4-(4-dimethylaminophenylazo)benzoyl; FAM=carboxyfluorescein)
Quantity:1mg net peptide.
Purity:≥95% (HPLC)
Appearance:Pink powder.
Solubility:Soluble in DMSO (≥10mM) or TRIS, pH 8.0 assay buffer (≥10μM).
Shipping:Blue Ice
Long Term Storage:-20°C
Use/Stability:Stock solutions in DMSO are stable for 3 months at -20°C.
Handling:Protect from light and moisture.
Regulatory Status:RUO - Research Use Only

Product Literature References

A substrate-optimized electrophoretic mobility shift assay for ADAM12: A. Kotzsch, et al.; Anal. Biochem. 452, 34 (2014), Abstract;
Catalytic properties of ADAM12 and its domain deletion mutants: J. Jacobsen, et al.; Biochemistry 47, 537 (2008), Abstract;
Heparan sulfate regulates ADAM12 through a molecular switch mechanism: H.P. Sorensen, et al.; J. Biol. Chem. 283, 31920 (2008), Abstract; Full Text
Fluorescent substrates for the proteinases ADAM17, ADAM10, ADAM8, and ADAM12 useful for high-throughput inhibitor screening: M.L. Moss & F.H. Rasmussen; Anal. Biochem. 366, 144 (2007), Abstract;

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