Diphosphopeptide with sequence, DHTGFLpTEpYVATR, corresponding to MAP kinase residues 177-189 inclusive of putative activation sites. Peptide can be used as a VHR phosphatase substrate (BML-SE333). Phosphate release may be quantified using BML-AK111 malachite green colorimetric reagent. Phosphate release is much more rapid for the phosphotyrosine residue compared to the phosphothreonine residue, both in vitro and in vivo.
Product Details
| Sequence: | Asp-His-Thr-Gly-Phe-Leu-pThr-Glu-pTyr-Val-Ala-Thr-Arg |
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| MW: | 1669.6 |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | White powder. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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