Fluorogenic substrate for angiotensin converting enzyme-1 (ACE1). The somatic form of ACE1 is a zinc-dependent peptidyl dipeptidase comprising two catalytic domains referred to as the N- and C-domains. The testicular form, due to a downstream start site, contains only the C-domain. Abz fluorescence is quenched by the Dnp group until cleavage (at Arg-Lys) separates them. Ex: 320 nm, Em: 420 nm. This quenched substrate is useful for assay of plasma, tissue, and cell culture ACE1 activity, inhibitor screening and kinetic analysis.
Product Details
| Alternative Name: | ACE1 substrate (fluorogenic) |
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| Sequence: | Abz-Phe-Arg-Lys(Dnp)-Pro-OH[Abz=o-aminobenzoicacid;Dnp=2,4-dinitrophenyl] |
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| MW: | 831.9 |
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| CAS: | 500799-61-1 |
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| Purity: | ≥95% |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Age-related reduction in brain ACE-2 is not exacerbated by Alzheimer’s disease pathology in mouse models of Alzheimer’s disease: R. MacLachlan, et al.; Aging Brain
3, 100062 (2023),
Abstract;
Activation of angiotensin-converting enzyme 2/angiotensin (1–7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling: R. Dang, et al.; Aging Cell
20, 13480 (2021),
Abstract;
Discovery of a Novel Metallo-β-Lactamase Inhibitor That Potentiates Meropenem Activity against Carbapenem-Resistant Enterobacteriaceae: M. Everett, et al.; Antimicrob. Agents Chemother.
62, e00074 (2018),
Abstract;
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Chymase-dependent production of angiotensin II: an old enzyme in old hearts: G. Froogh, et al.; Am. J. Physiol. Heart Circ. Physiol.
312, H223 (2017),
Abstract;
