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Inhibitor of non-muscle myosin II ATPase
BML-EI315-0005 5 mg 161.00 USD
BML-EI315-0025 25 mg 637.00 USD
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  • Rapid and selective inhibitor of Myosin II ATPase
  • Used in cardiovascular research
  • Highly cited
Blebbistatin (so named because of its ability to block cell blebbing) is a rapid, selective, and high-affinity (IC50 approx. 4 µM) inhibitor of non-muscle myosin II ATPase activity. During cell division, blebbistatin inhibits contraction of the cleavage furrow without disrupting mitosis. Blebbistatin impedes cell migration and cytokinesis. Blebbistatin also reduces infiltration of inflammatory cells, inhibits pancreatic adenocarcinoma cellular invasiveness, PDGF-stimulated fibroblast-collagen matrix contraction, and diminishes harm to vascular system in models of arterial thrombosis in vivo.

Product Details

Alternative Name:(±)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one, (±)-Blebbistatin
Purity:≥97% (HPLC)
Identity:Identity determined by NMR.
Appearance:Bright yellow solid.
Solubility:Soluble in DMSO (25mg/ml).
Shipping:Ambient Temperature
Long Term Storage:-20°C
Use/Stability:Exposure to blue light (i.e. 450-490nm) may lead to the degradation and inactivation of blebbistatin via cytotoxic intermediates, which may be a source of concern when used in fluorescence applications.
Regulatory Status:RUO - Research Use Only
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Product Literature References

Statins Protect Against Early Stages of Doxorubicin-induced Cardiotoxicity Through the Regulation of Akt Signaling and SERCA2: K. Dadson, et al.; CJC open 4, 1043 (2022), Abstract;
Synchronizing systolic calcium release with azumolene in an experimental model: D. Si, et al.; Heart Rhythm O2 3, 568 (2022), Abstract;
Anti-arrhythmic and inotropic effects of empagliflozin following myocardial ischemia: M.A. Azam, eta al.; Life Sci. 276, 119440 (2021), Abstract;
LFA-1 and Kindlin-3 enable the collaborative transport of SLP-76 microclusters by myosin and dynein motors: K.P. Eidell, et al.; J. Cell Sci. 36, 258602 (2021), Abstract;
Probing TGF-β1-induced cytoskeletal rearrangement by fluorescent-labeled silica nanoparticle uptake assay: H.R. Shin, et al.; Biochem. Biophys. Rep. 28, 101137 (2021), Abstract;
Osmotic gradients induce stable dome morphogenesis on extracellular matrix: S. Ishida-Ishihara, et al.; J. Cell Sci. 133, jcs243865 (2020), Abstract;
Actomyosin contractility scales with myoblast elongation and enhances differentiation through YAP nuclear export: C. Bruyere, et al.; Sci. Rep. 9, 15565 (2019), Abstract; Full Text
Omegasome-proximal PtdIns(4,5)P2 couples F-actin mediated mitoaggregate disassembly with autophagosome formation during mitophagy: C.W. Hsieh, et al.; Nat. Commun. 10, 969 (2019), Application(s): added during cell culture to screen for functional and structrual changes in mitophagy in HeLa cells expressing EBFP-2-Parkin, EGFP-dMito and KillerRed-dMito, Abstract; Full Text
Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules.: M. Martin, et al.; Elife 7, e33864 (2018), Abstract; Full Text
Tropomyosin isoform Tpm2.1 regulates collective and amoeboid cell migration and cell aggregation in breast epithelial cells: H. Shin, et al.; Oncotarget 56, 95192 (2017), Abstract; Full Text
Myosin VI regulates gene pairing and transcriptional pause release in T cells.: C.E. Zorca, et al.; PNAS 13, E1587 (2015), Abstract; Full Text
Opposite rheological properties of neuronal microcompartments predict axonal vulnerability in brain injury: T. Grevesse, et al.; Sci. Rep. 5, 9475 (2015), Application(s): Cell Culture, Abstract; Full Text
Distinct roles for ROCK1 and ROCK2 in the regulation of cell detachment: J. Shi, et al.; Cell Death Dis. 4, e483 (2013), Abstract; Full Text
The nucleus of endothelial cell as a sensor of blood flow direction: E. Tkachenko, et al.; Biol. Open 2, 1007 (2013), Abstract; Full Text
Inhibition of pancreatic adenocarcinoma cellular invasiveness by blebbistatin: a novel myosin II inhibitor: M.S. Duxbury, et al.; Biochem. Biophys. Res. Commun. 313, 992 (2004), Abstract;
Mechanism of blebbistatin inhibition of myosin II: M. Kovacs, et al.; J. Biol. Chem. 279, 35557 (2004), Abstract; Full Text
Different molecular motors mediate platelet-derived growth factor and lysophosphatidic acid-stimulated floating collagen matrix contraction: M. Abe, et al.; J. Biol. Chem. 278, 47707 (2003), Abstract; Full Text
Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor: A.F. Straight, et al.; Science 299, 1743 (2003), Abstract;

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