Blebbistatin (so named because of its ability to block cell blebbing) is a selective and high-affinity (IC50 approx. 4 µM) inhibitor of non-muscle myosin II. During cell division blebbistatin inhibits contraction of the cleavage furrow without disrupting mitosis. It inhibits pancreatic adenocarcinoma cellular invasiveness and PDGF-stimulated fibroblast-collagen matrix contraction.
Product Specification
| Formula: | C18H16N2O2 |
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| MW: | 292.3 |
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| CAS: | 674289-55-5 |
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| Purity: | ≥97% |
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| Identity: | Determined by NMR. |
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| Appearance: | Bright yellow solid. |
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| Solubility: | Soluble in DMSO (25mg/ml). |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Abstract;
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Application(s): added during cell culture to screen for functional and structrual changes in mitophagy in HeLa cells expressing EBFP-2-Parkin, EGFP-dMito and KillerRed-dMito,
Abstract;
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Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules.: M. Martin, et al.; Elife
7, e33864 (2018),
Abstract;
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Tropomyosin isoform Tpm2.1 regulates collective and amoeboid cell migration and cell aggregation in breast epithelial cells: H. Shin, et al.; Oncotarget
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Abstract;
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Myosin VI regulates gene pairing and transcriptional pause release in T cells.: C.E. Zorca, et al.; PNAS
13, E1587 (2015),
Abstract;
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Opposite rheological properties of neuronal microcompartments predict axonal vulnerability in brain injury: T. Grevesse, et al.; Sci. Rep.
5, 9475 (2015),
Application(s): Cell Culture,
Abstract;
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Distinct roles for ROCK1 and ROCK2 in the regulation of cell detachment: J. Shi, et al.; Cell Death Dis.
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Abstract;
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The nucleus of endothelial cell as a sensor of blood flow direction: E. Tkachenko, et al.; Biol. Open
2, 1007 (2013),
Abstract;
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Inhibition of pancreatic adenocarcinoma cellular invasiveness by blebbistatin: a novel myosin II inhibitor: M.S. Duxbury, et al.; Biochem. Biophys. Res. Commun.
313, 992 (2004),
Abstract;
Mechanism of blebbistatin inhibition of myosin II: M. Kovacs, et al.; J. Biol. Chem.
279, 35557 (2004),
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Different molecular motors mediate platelet-derived growth factor and lysophosphatidic acid-stimulated floating collagen matrix contraction: M. Abe, et al.; J. Biol. Chem.
278, 47707 (2003),
Abstract;
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Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor: A.F. Straight, et al.; Science
299, 1743 (2003),
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