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CV-Caspase 3 & 7 detection kit

BML-AK118-0001 1 Kit 317.00 USD
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A consistant feature of mammalian cell apoptosis is the activation of caspases 3 and/or 7. Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy. When cleaved by caspase 3 or 7, the mono and unsubstituted cresyl violet fluoresce when excited at 550-590 nm. Once cells are grown, and apoptosis induced, staining is completed in just a few minutes. Hoechst 33342 and Acridine Orange are also included to allow visualization of nuclei and lysosomes.

Product Details

Applications:Fluorescence microscopy, Fluorescent detection
Handling:Store the unopened kit and each unopened component at 2-8°C until the expiration date. Once reconstituted with DMSO, use CR(DEVD)2 immediately, or store at ≤-20°C up to 6 months, protected from light and thawed no more than twice during that time.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
Contents:CR(DEVD)2 reagent, Acridine orange, Hoechst 33342 stain.
Regulatory Status:RUO - Research Use Only

Product Literature References

Effects of mutations and deletions in the human optineurin gene: S. Turturro, et al.; Springerplus 3, 99 (2014), Abstract; Full Text
Processing of optineurin in neuronal cells: X. Shen, et al.; J. Biol. Chem. 286, 3618 (2011), Abstract; Full Text
Differential effects of myocilin and optineurin, two glaucoma genes, on neurite outgrowth: T. Koga, et al.; Am. J. Pathol. 176, 343 (2010), Abstract; Full Text

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