A consistant feature of mammalian cell apoptosis is the activation of caspases 3 and/or 7. Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy. When cleaved by caspase 3 or 7, the mono and unsubstituted cresyl violet fluoresce when excited at 550-590 nm. Once cells are grown, and apoptosis induced, staining is completed in just a few minutes. Hoechst 33342 and Acridine Orange are also included to allow visualization of nuclei and lysosomes.
Product Details
| Applications: | Fluorescence microscopy, Fluorescent detection
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| Handling: | Store the unopened kit and each unopened component at 2-8°C until the expiration date. Once reconstituted with DMSO, use CR(DEVD)2 immediately, or store at ≤-20°C up to 6 months, protected from light and thawed no more than twice during that time. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | +4°C |
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| Contents: | CR(DEVD)2 reagent, Acridine orange, Hoechst 33342 stain. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Effects of mutations and deletions in the human optineurin gene: S. Turturro, et al.; Springerplus
3, 99 (2014),
Abstract;
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Processing of optineurin in neuronal cells: X. Shen, et al.; J. Biol. Chem.
286, 3618 (2011),
Abstract;
Full Text
Differential effects of myocilin and optineurin, two glaucoma genes, on neurite outgrowth: T. Koga, et al.; Am. J. Pathol.
176, 343 (2010),
Abstract;
Full Text
