Replaces Prod. #: ADI-ADK-700
FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm, Em(max): 530nm).
Typical flow cytometry results. The Annexin V-FITC apoptosis detection kit allows the detection and discrimination between apoptotic and necrotic cells.
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Product Details
| Applications: | Flow Cytometry, Fluorescence microscopy, Fluorescent detection
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| Application Notes: | For the detection of apoptotic cells by flow cytometry or microscopy |
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| Purity: | ≥98% (SDS-PAGE; HPLC) |
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| Handling: | After opening annexin V-FITC, aliquot contents and freeze at -20°C. Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | +4°C |
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| Contents: | Annexin V-FITC: 100 tests/500µl; 300 tests/1.5ml;Liquid. In 50mM TRIS, pH 7.4, 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
Binding Buffer: 50ml (4x concentrated)Concentration after dilution: 10mM HEPES/NaOH, pH 7.4, 140mM sodium chloride, 2.5mM CaCl2.
Propidium Iodide: 20µg/ml (100 tests: 1.6ml, 300 tests: 2 x 1.6ml). |
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| Protocol: | Flow Cytometry Protocol:
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Dilute binding buffer 1:4 in distilled water (50ml binding buffer and 150ml water)
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Brief centrifugation of annexin V-FITC before use is recommended
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Wash cells in PBS by gentle shaking or pipetting up and down.
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Resuspend cells in prediluted binding buffer; adjust cell density to 2-5x105/ml
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Take 195µl cell suspension and add 5µl annexin V-FITC
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Mix and incubate 10 minutes in the dark at room temperature
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Wash cells 1x with PBS and resuspend in 190µl prediluted binding buffer
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Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)
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FACS analysis
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Nigrosporins B, a Potential Anti-Cervical Cancer Agent, Induces Apoptosis and Protective Autophagy in Human Cervical Cancer Ca Ski Cells Mediated by PI3K/AKT/mTOR Signaling Pathway: J. Zhang, et al.; Molecules
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Mitochondrial dependent pathway is involved in the protective effects of carboxymethylated chitosan on nitric oxide-induced apoptosis in chondrocytes: B. He, et al.; BMC Complement Med. Ther.
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Radiotherapy in combination with hyperthermia suppresses lung cancer progression via increased NR4A3 and KLF11 expression: B. Son, et al.; Int. J. Radiat. Biol.
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Targeting Mitochondrial Oxidative Phosphorylation Abrogated Irinotecan Resistance in NSCLC: S. Lee, et al.; Sci. Rep.
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Radio-protective effect and mechanism of 4-Acetamido-2,2,6,6- tetramethylpiperidin-1-oxyl in HUVEC cells: F. Wang, et al.; Environ. Health Prev. Med.
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Effects of the pulsed electromagnetic field PST® on human tendon stem cells: a controlled laboratory study: P. Randelli, et al.; BMC Complement. Altern. Med.
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Application(s): Cell apoptosis analysis, PST and control cells,
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Nanoparticles of barium induce apoptosis in human phagocytes: L. Mores, et al.; Int. J. Nanomedicine
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Application(s): Flow cytometry apoptosis assay with human cells,
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Full Text
Protective Effects of N-Acetyl-L-Cysteine in Human Oligodendrocyte Progenitor Cells and Restoration of Motor Function in Neonatal Rats with Hypoxic-Ischemic Encephalopathy: D. Park, et al.; Evid. Based Complement. Alternat. Med.
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Application(s): Flow Cytometry,
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GX15-070 (Obatoclax) Induces Apoptosis and Inhibits Cathepsin D- and L–Mediated Autophagosomal Lysis in Antiestrogen-Resistant Breast Cancer Cells : J.L. Schwartz-Roberts, et al.; Mol. Cancer Ther.
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Glucose-Regulated Protein 78 Controls Cross-talk between Apoptosis and Autophagy to Determine Antiestrogen Responsiveness: K. Cook, et al.; Cancer Res.
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Selective FLT3 inhibition of FLT3-ITD+ acute myeloid leukaemia resulting in secondary D835Y mutation: a model for emerging clinical resistance patterns: A. Moore, et al.; Leukemia
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Specific binding of hypochlorite-oxidized HDL to platelet CD36 triggers proinflammatory and procoagulant effects: A. Assinger, et al.; Atherosclerosis
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