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Annexin V-FITC apoptosis detection kit

Easy, quick assay to distinguish apoptotic cells vs necrotic cells
ALX-850-020-KI02 100 tests 458.00 USD
ALX-850-020-KI01 300 tests 689.00 USD
Do you need bulk/larger quantities?
Replaces Prod. #: ADI-ADK-700

FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm, Em(max): 530nm).
Annexin V-FITC apoptosis detection kit Flow Cytometry
Typical flow cytometry results. The Annexin V-FITC apoptosis detection kit allows the detection and discrimination between apoptotic and necrotic cells.
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Annexin V-FITC apoptosis detection kit Flow Cytometry

Product Details

Applications:Flow Cytometry, Fluorescence microscopy, Fluorescent detection
Application Notes:For the detection of apoptotic cells by flow cytometry or microscopy
Purity:≥98% (SDS-PAGE; HPLC)
Handling:After opening annexin V-FITC, aliquot contents and freeze at -20°C. Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
Contents:Annexin V-FITC: 100 tests/500µl; 300 tests/1.5ml;Liquid. In 50mM TRIS, pH 7.4, 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
Binding Buffer: 50ml (4x concentrated)Concentration after dilution: 10mM HEPES/NaOH, pH 7.4, 140mM sodium chloride, 2.5mM CaCl2.
Propidium Iodide: 20µg/ml (100 tests: 1.6ml, 300 tests: 2 x 1.6ml).
Protocol:Flow Cytometry Protocol:
  1. Dilute binding buffer 1:4 in distilled water (50ml binding buffer and 150ml water)
  2. Brief centrifugation of annexin V-FITC before use is recommended
  3. Wash cells in PBS by gentle shaking or pipetting up and down.
  4. Resuspend cells in prediluted binding buffer; adjust cell density to 2-5x105/ml
  5. Take 195µl cell suspension and add 5µl annexin V-FITC
  6. Mix and incubate 10 minutes in the dark at room temperature
  7. Wash cells 1x with PBS and resuspend in 190µl prediluted binding buffer
  8. Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)
  9. FACS analysis
Regulatory Status:RUO - Research Use Only

Product Literature References

Mitochondrial dependent pathway is involved in the protective effects of carboxymethylated chitosan on nitric oxide-induced apoptosis in chondrocytes: B. He, et al.; BMC Complement Med. Ther. 20, 23 (2020), Abstract; Full Text
Radiotherapy in combination with hyperthermia suppresses lung cancer progression via increased NR4A3 and KLF11 expression: B. Son, et al.; Int. J. Radiat. Biol. 95, 1696 (2019), Abstract;
Targeting Mitochondrial Oxidative Phosphorylation Abrogated Irinotecan Resistance in NSCLC: S. Lee, et al.; Sci. Rep. 8, 15707 (2018), Abstract; Full Text
Radio-protective effect and mechanism of 4-Acetamido-2,2,6,6- tetramethylpiperidin-1-oxyl in HUVEC cells: F. Wang, et al.; Environ. Health Prev. Med. 22, 14 (2017), Abstract; Full Text
Effects of the pulsed electromagnetic field PST® on human tendon stem cells: a controlled laboratory study: P. Randelli, et al.; BMC Complement. Altern. Med. 16, 293 (2016), Application(s): Cell apoptosis analysis, PST and control cells, Abstract; Full Text
Nanoparticles of barium induce apoptosis in human phagocytes: L. Mores, et al.; Int. J. Nanomedicine 10, 6021 (2015), Application(s): Flow cytometry apoptosis assay with human cells, Abstract; Full Text
Protective Effects of N-Acetyl-L-Cysteine in Human Oligodendrocyte Progenitor Cells and Restoration of Motor Function in Neonatal Rats with Hypoxic-Ischemic Encephalopathy: D. Park, et al.; Evid. Based Complement. Alternat. Med. 2015, 764251 (2015), Application(s): Flow Cytometry, Abstract; Full Text
GX15-070 (Obatoclax) Induces Apoptosis and Inhibits Cathepsin D- and L–Mediated Autophagosomal Lysis in Antiestrogen-Resistant Breast Cancer Cells : J.L. Schwartz-Roberts, et al.; Mol. Cancer Ther. 12, 448 (2013), Abstract;
Glucose-Regulated Protein 78 Controls Cross-talk between Apoptosis and Autophagy to Determine Antiestrogen Responsiveness: K. Cook, et al.; Cancer Res. 72, 3337 (2012), Abstract; Full Text
Selective FLT3 inhibition of FLT3-ITD+ acute myeloid leukaemia resulting in secondary D835Y mutation: a model for emerging clinical resistance patterns: A. Moore, et al.; Leukemia 26, 1462 (2012), Abstract; Full Text
Specific binding of hypochlorite-oxidized HDL to platelet CD36 triggers proinflammatory and procoagulant effects: A. Assinger, et al.; Atherosclerosis 212, 153 (2010), Abstract;

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