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TRAIL, soluble (human) (recombinant) set

ALX-850-018-KI01 1 Set 739.00 USD
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Product Details

Alternative Name:Apo-2L, TNFSF 10, CD253
Application Notes:Induces apoptosis in a concentration range of 1-100ng/ml if applied with the cross-linking enhancer.
Species reactivity:Human, Mouse
Specificity:TRAIL binds to human and mouse TRAIL receptors and human osteoprotegerin (OPG).
Specific Activity:ED50: 10ng/ml (Jurkat cells)
Purity:≥95% (SDS-PAGE)
Contents:10µg of TRAIL, Soluble (human) (recombinant) (Prod. No. ALX-522-003) and 2x50µg of Enhancer for Ligands (Prod. No. ALX-804-034).
Formulation:Lyophilized. Contains PBS.
Reconstitution:Reconstitute TRAIL with 100µl and each vial of enhancer with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Concentration:TRAIL: 0.1mg/ml after reconstitution
Enhancer: 1mg/ml after reconstitution
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Shipping:Blue Ice
Long Term Storage:-20°C
Source:Produced in E. coli. The extracellular domain of human TRAIL (Apo-2L) (aa 95-281) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
Technical Info/Product Notes:FLAG is a registered trademark of Sigma-Aldrich Co.
UniProt ID:P50591
Regulatory Status:RUO - Research Use Only

Product Literature References

Q482H mutation of procaspase-8 in acute myeloid leukemia abolishes caspase-8-mediated apoptosis by impairing procaspase-8 dimerization: M. Li, et al.; Biochem. Biophys. Res. Commun. 495, 1376 (2018), Abstract;
Human mast cells undergo TRAIL-induced apoptosis: B. Berent-Maoz, et al.; J. Immunol. 176, 2272 (2006), Abstract;
Chemotherapy enhances TNF-related apoptosis-inducing ligand DISC assembly in HT29 human colon cancer cells: S. Lacour, et al.; Oncogene 22, 1807 (2003), Abstract;
Death ligand TRAIL induces no apoptosis but inhibits activation of human (auto)antigen-specific T cells: J.D. Lunemann, et al.; J. Immunol. 168, 4881 (2002), Abstract;
Death receptors bind SHP-1 and block cytokine-induced anti-apoptotic signaling in neutrophils: I. Daigle, et al.; Nat. Med. 8, 61 (2002), Abstract;
Induction of TRAIL-mediated glioma cell death by human T cells: J. Dörr, et al.; J. Neuroimmunol. 122, 117 (2002), Abstract;
Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Signaling in Activated T Cells Abrogates TRAIL-Induced Apoptosis Upstream of the Mitochondrial Amplification Loop and Caspase-8: T.S. Soderstrom, et al.; J. Immunol. 169, 2851 (2002), Abstract;
Requirement of BAX for TRAIL/Apo2L-induced apoptosis of colorectal cancers: synergism with sulindac-mediated inhibition of Bcl-x(L): R. Ravi & A. Bedi; Cancer Res. 62, 1583 (2002), Abstract;
TRAIL and its receptors in the colonic epithelium: a putative role in the defense of viral infections: J. Strater, et al.; Gastroenterology 122, 659 (2002), Abstract;
TRAIL/Apo-2 ligand induces primary plasma cell apoptosis: J. Ursini-Siegel, et al.; J. Immunol. 169, 5505 (2002), Abstract; Full Text
Tumor necrosis factor-related apoptosis-inducing ligand-induced death-inducing signaling complex and its modulation by c-FLIP and PED/PEA-15 in glioma cells: C. Xiao, et al.; J. Biol. Chem. 277, 25020 (2002), Abstract; Full Text
A central role for death receptor-mediated apoptosis in the rejection of tumors by NK cells: V. Screpanti, et al.; J. Immunol. 167, 2068 (2001), Abstract; Full Text
Caspase-10 is an initiator caspase in death receptor signaling: J. Wang, et al.; PNAS 98, 13884 (2001), Abstract; Full Text
NF-κB signals induce the expression of c-FLIP: O. Micheau, et al.; Mol. Cell. Biol. 21, 5299 (2001), Abstract; Full Text
The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines: Q. Liao, et al.; FEBS Lett. 503, 151 (2001), Abstract;
Three adenovirus E3 proteins cooperate to evade apoptosis by tumor necrosis factor-related apoptosis-inducing ligand receptor-1 and -2: C.A. Benedict et al.; J. Biol. Chem. 276, 3270 (2001), Abstract; Full Text
Sensitivity to TRAIL/APO-2L-mediated apoptosis in human renal cell carcinomas and its enhancement by topotecan: M. Dejosez, et al.; Cell Death Differ. 7, 1127 (2000), Abstract; Full Text

General Literature References

Cell death: TRAIL and its receptors: P. Golstein; Curr. Biol. 7, R750 (1997), Abstract;
How TRAIL kills cancer cells, but not normal cells: T. Gura; Science 277, 768 (1997), Abstract;
Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family: R.M. Pitti, et al.; J. Biol. Chem. 271, 12687 (1996), Abstract;
Identification and characterization of a new member of the TNF family that induces apoptosis: S.R. Wiley, et al.; Immunity 3, 673 (1995), Abstract;

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