Product Details
Alternative Name: | Lymphocyte activation gene-3, FDC protein, CD223 |
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Clone: | 11E3 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Recombinant human LAG-3 (lymphocyte activation gene-3). |
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UniProt ID: | P18627 |
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Source: | Purified from the hybridoma culture supernatant by Protein G Fast Flow Column. |
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Species reactivity: | Human Monkey
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Specificity: | Recognizes the first N-terminal D1 domain of LAG-3. |
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Applications: | ELISA, Flow Cytometry, ICC, IHC (FS), IP, WB, FUNC
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Recommended Dilutions/Conditions: | ELISA (capture) Immunoprecipitation (10µg/ml) Western Blot (5µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Formulation: | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Scientific Background: | The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses. |
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Regulatory Status: | RUO - Research Use Only |
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Flow cytometry analysis of 10^6 MCF-7 cells stained using LAG-3 (human) monoclonal antibody (11E3), (Prod. No. ALX-804-805), at a concentration of 50µg/mL.
SDS-PAGE analysis of ALX-804-805: Lane 1: MW Marker, Lane 2 and Lane 3: 2µg of LAG-3 (human) monoclonal antibody (11E3), resolved under reduced and non-reduced condition.
Western blot analysis of LAG-3 mAb (11E3) (Prod. No. ALX-804-805): Lane 1: MW marker, Lane 2: LAG-3 (human):Fc (human), (recombinant) (Prod. No. ALX-522-078).
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Product Literature References
Elevated expression of LAG-3, but not PD-1, is associated with impaired iNKT cytokine production during chronic HIV-1 infection and treatment : J.A. Juno, et al.; Retrovirology
12, 17 (2015),
Application(s): ELISA,
Abstract;
Full Text
A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors: F. Triebel, et al.; Cancer Lett.
235, 147 (2006),
Abstract;
Characterization of the major histocompatibility complex class II binding site on LAG-3 protein: B. Huard, et al.; PNAS
94, 5744 (1997),
Abstract;
Full Text
Cellular expression and tissue distribution of the human LAG-3-encoded protein, an MHC class II ligand: B. Huard, et al.; Immunogenetics
39, 213 (1994),
Abstract;