Product Details
| Alternative Name: | TLR9, CD289 |
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| Clone: | 26C593.2 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9). |
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| UniProt ID: | Q9NR96 |
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| Species reactivity: | Human, Mouse, Rat
Dog, Horse, Monkey
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| Applications: | Flow Cytometry, IHC (FS), IHC (PS), WB
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| Recommended Dilutions/Conditions: | Flow Cytometry (intracellular, 0.1-2µg/106 cells)
Immunohistochemistry (frozen sections, 10-20µg/ml)
Western Blot (1-5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Flow Cytometry: Suitable for cell surface and intracellular FC.
Detects a band of ~120kDa by Western blot, may also detect a smaller band of TLR9 isoform B. |
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| Positive Control: | Whole cell lysate from human peripheral blood monocyte cells (PBMC) or Daudi can be used as positive control. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis: Formalin-fixed, paraffin-embedded human spleen probed with Toll-like receptor 9, mAb (26C593.2) at 5µg/ml.
Figure 1: Western blot analysis of TLR9 using MAb to Toll-like Receptor 9 (human) (26C593.2) (Prod. No.
ALX-804-364) at a dilution of 3µg/ml. A) human PBMC; B) human intestine; C) mouse intestine; D) rat intestine lysate; E) human MCF7 cell lysate (MAb dilution 5μg/ml)."
Figure 2: Intracellular flow cytometric analysis of TLR9 in Ramos cells using 0.1μg MAb to Toll-like Receptor 9 (human) (26C593.2) (Prod. No.
ALX-804-364). Shaded histogram represents Ramos cells without antibody; green represents isotype control (mouse IgG1); red represents MAb to Toll-like Receptor 9 (human) (26C593.2)."
Figure 3: Immunohistochemical analysis of TLR9 in A) adenocarcinoma of the lung, C) A549 cells, and F) malignant lung tissues using MAb to Toll-like Receptor 9 (human) (26C593.2) (Prod. No.
ALX-804-364) at 1:100 dilution, (600x magnification)."
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Product Literature References
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils: C.K. Wong, et al.; Am. J. Respir. Cell Mol. Biol.
37, 85 (2007),
Abstract;
Surface expression of Toll-like receptor 9 is upregulated on intestinal epithelial cells in response to pathogenic bacterial DNA: J.B. Ewaschuk, et al.; Infect. Immun.
75, 2572 (2007),
Abstract;
Activation of anti-hepatitis C virus responses via Toll-like receptor 7: J. Lee, et al.; PNAS
103, 1828 (2006),
Abstract;
Full Text
The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7 and 9: K. Tabeta, et al.; Nat. Immunol.
7, 156 (2006),
Abstract;
Full Text
Toll-like receptor 9 (TLR9) is present in murine liver sinusoidal endothelial cells (LSECs) and mediates the effect of CpG-oligonucleotides: M. Martin-Armas, et al.; J. Hepatol.
44, 939 (2006),
Abstract;
Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis: A. Mansson, et al.; Respir Res.
7, 36 (2006),
Abstract;
Full Text
Evidence of Toll-like receptor molecules on human platelets: F. Cognasse, et al.; Immunol. Cell Biol.
83, 196 (2005),
Abstract;
Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line: M.D. Srivastava and B.I. Srivastava; Leuk. Res.
29, 813 (2005),
Abstract;
Human lung cancer cells express functionally active Toll-like receptor 9: D. Droemann, et al.; Respir. Res.
6, 1 (2005),
Abstract;
Full Text
TGF-alpha regulates TLR expression and function on epidermal keratinocytes: L.S. Miller, et al.; J. Immunol.
174, 6137 (2005),
Abstract;
TLR-induced inflammation in cystic fibrosis and non-cystic fibrosis airway epithelial cells: C.M. Greene, et al.; J. Immunol.
174, 1638 (2005),
Abstract;
Full Text
CpG oligodeoxynucleotides activate HIV replication in latently infected human T cells: C. Scheller, et al.; J. Biol. Chem.
279, 21897 (2004),
Abstract;
Full Text
Cytoplasmic domain-mediated dimerizations of toll-like receptor 4 observed by beta-lactamase enzyme fragment complementation: H.K. Lee, et al.; J. Biol. Chem.
279, 10564 (2004),
Abstract;
Full Text
