Replaces Prod. #: ADI-905-666
Product Details
Alternative Name: | Shc transforming protein 1, SH2 domain protein C1, Shc1 |
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Clone: | 1E3 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to aa 236-243 (D236HQpYpYNDF243) of shc phosphorylated at Tyr239 and Tyr240 conjugated to KLH. |
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UniProt ID: | P29353 |
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Species reactivity: | Human, Mouse, Rat Dog
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Specificity: | Recognizes p46shc, p52shc and p66shc when phosphorylated at Tyr239/240. |
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Crossreactivity: | Does not cross-react with non-phosphorylated shc. |
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Applications: | ELISA, IHC, WB
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Recommended Dilutions/Conditions: | Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Positive Control: | Included. (Prod. No. ALX-840-025) |
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Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
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Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
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Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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