| ALX-804-357-C100 | 100 µg | 477.00 USD |  |
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| Alternative Name: | Shc transforming protein 1, SH2 domain protein C1, Shc1 |
| Clone: | 1E3 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to aa 236-243 (D236HQpYpYNDF243) of shc phosphorylated at Tyr239 and Tyr240 conjugated to KLH. |
| UniProt ID: | P29353 |
| Species reactivity: | Human, Mouse, Rat Dog |
| Specificity: | Recognizes p46shc, p52shc and p66shc when phosphorylated at Tyr239/240. |
| Crossreactivity: | Does not cross-react with non-phosphorylated shc. |
| Applications: | ELISA, IHC, WB |
| Recommended Dilutions/Conditions: | Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Positive Control: | Included. (Prod. No. ALX-840-025) |
| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Regulatory Status: | RUO - Research Use Only |
