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Ceramide monoclonal antibody (MID 15B4)

ALX-804-196-T050 50 tests 552.00 USD
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Product Specification

Clone:MID 15B4
Immunogen:Ceramide (sphingosine-[trans-D-erythro-2-amino-4-octadecene-1,3-diol]) conjugated to BSA.
Source:Purified from ascites by gel filtration on sephacryl S-300.
Species reactivity:Species independent
Specificity:Recognizes C16- and C24-ceramide, dihydroceramide, sphingomyelin and phosphatidylcholine in highly artificial lipid overlay test systems. Under more physiological in vitro and in vivo conditions highly specific for ceramide and does not cross-react with sphingomyelin, cholesterol or other phospholipids.
Applications:ELISA, Flow Cytometry, ICC, IHC (PS)
Recommended Dilutions/Conditions:ELISA (1:10)
Immunohistochemistry (1:10)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Quantity:1ml (50 tests). Suggested amount: 20µl/test.
Formulation:Liquid. In PBS, pH 7.2, containing 0.5M sodium chloride, 0.1% BSA and 0.09% sodium azide.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
Technical Info/Product Notes:Cited samples:
For an overview on cited samples please click here.
Regulatory Status:RUO - Research Use Only

Product Literature References

Acid sphingomyelinase promotes SGK1-dependent vascular calcification: T. Luong, et al.; Clin. Sci. (2021), Abstract;
Medial calcification in the arterial wall of smooth muscle cell‐specific Smpd1 transgenic mice: A ceramide‐mediated vasculopathy: O.M. Bhat, et al.; J. Cell. Mol. Med. 24, 539 (2020), Abstract; Full Text
Apurinic/apyrimidinic endonuclease/redox factor 1 (APE1) alleviates myocardial hypoxia-reoxygenation injury by inhibiting oxidative stress and ameliorating mitochondrial dysfunction: J. Hao, et al.; Exp. Ther. Med. 17, 2143 (2019), Abstract; Full Text
Autophagy augmentation alleviates cigarette smoke-induced CFTR-dysfunction, ceramide-accumulation and COPD-emphysema pathogenesis: M. Bodas, et al.; Free Radic. Biol. Med. 131, 81 (2019), Application(s): Co-staining and fluorescence microscopy using human and murine lung tissue sections, Abstract;
Comparison of ceramide retention in the stratum corneum between dry skin and normal skin using animal model with fluorescent imaging method: M. Aoki, et al.; Skin Res. Technol. 25, 158 (2019), Abstract;
Mechanism of palmitic acid-induced deterioration of in vitro development of porcine oocytes and granulosa cells: H. Shibahara, et al.; Theriogenology 141, 54 (2019), Abstract;
Novel sphingomyelin biomarkers for brain glioma and associated regulation research on the PI3K/Akt signaling pathway: X.H. Zhai, et al.; Oncol. Lett. 18, 6207 (2019), Abstract; Full Text
Rab25 Deficiency Perturbs Epidermal Differentiation and Skin Barrier Function in Mice: H. Jeong, et al.; Biomol. Ther. (Seoul) 27, 553 (2019), Abstract; Full Text
Heterotrimeric G-protein subunit Gαi2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets: H. Cao, et al.; Physiol. Rep. 6, 13841 (2018), Abstract; Full Text
Nuclear phosphatidylinositol 4,5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription: M. Sobol, et al.; J. Cell Sci. 131, jcs211094 (2018), Abstract;
Palmitic acid induces ceramide accumulation, mitochondrial protein hyper-acetylation and mitochondrial dysfunction in porcine oocytes: N. Itami, et al.; Biol. Reprod. 98, 644 (2018), Abstract;
Pyridostigmine protects against cardiomyopathy associated with adipose tissue browning and improvement of vagal activity in high-fat diet rats: Y. Lu, et al.; Biochim. Biophys. Acta 1864, 1037 (2018), Abstract;
Stress-induced host membrane remodeling protects from infection by non-motile bacterial pathogens: C. Tawk, et al.; EMBO J. 37, e98529 (2018), Abstract; Full Text
Defective Sphingosine-1-phosphate metabolism is a druggable target in Huntington's disease: A. Di Pardo, et al.; Sci. Rep. 7, 5280 (2017), Abstract; Full Text
Inhibition of Suicidal Erythrocyte Death by Volasertib: A. Al Mamun Bhuyan, et al.; Cell. Physiol. Biochem. 43, 1 (2017), Abstract; Full Text
Wash or wipe? A comparative study of skin physiological changes between water washing and wiping after skin cleaning: K. Ogai, et al.; Skin Res. Technol. 23, 519 (2017), Abstract;
Anidulafungin-Induced Suicidal Erythrocyte Death: T. Peter, et al.; Cell. Physiol. Biochem. 38, 2272 (2016), Application(s): Flow cytometry, Abstract; Full Text
Bile Acid-Induced Suicidal Erythrocyte Death: E. Lang, et al.; Cell Physiol. Biochem. 38, 1500 (2016), Application(s): Cell culture, Abstract; Full Text
Enhanced Eryptosis Following Exposure to Dolutegravir: A. Al Mamun Bhuyan, et al.; Cell Physiol. Biochem. 39, 639 (2016), Application(s): Ceramide abundance detection, by flow cytometric analysis, Abstract; Full Text
Estrogen Metabolite 16α-Hydroxyestrone Exacerbates Bone Morphogenetic Protein Receptor Type II-Associated Pulmonary Arterial Hypertension Through MicroRNA-29-Mediated Modulation of Cellular Metabolism: X. Chen, et al.; Circulation 133, 82 (2016), Application(s): Immunostaining, Abstract; Full Text
Galectin-1 is a local but not systemic immunomodulatory factor in mesenchymal stromal cells: R. Fajka-Boja, et al.; Cytotherapy 18, 360 (2016), Application(s): Cell staining, Abstract;
Micafungin-Induced Suicidal Erythrocyte Death: T. Peter, et al.; Cell Physiol. Biochem. 39, 584 (2016), Application(s): Determine ceramide abundance at the erythrocyte surface, Abstract; Full Text
Nocodazole Induced Suicidal Death of Human Erythrocytes: E. Signoretto, et al.; Cell. Physiol. Biochem. 38, 379 (2016), Application(s): Cell staining, Abstract; Full Text
oxLDL and eLDL Induced Membrane Microdomains in Human Macrophages: S. Wallner, et al.; PLoS One 11, e0166798 (2016), Abstract; Full Text
Stimulated Suicidal Erythrocyte Death in Arteritis: R. Bissinger, et al.; Cell. Physiol. Biochem. 39, 1068 (2016), Application(s): Determination of ceramide formation, erythrocytes, Abstract; Full Text
Stimulating Effect of Sclareol on Suicidal Death of Human Erythrocytes: E. Signoretto, et al.; Cell Physiol. Biochem. 39, 554 (2016), Application(s): Determination of Ceramide abundance, Abstract; Full Text
Stimulation of Suicidal Erythrocyte Death by Ceritinib-Treatment of Human Erythrocytes: A.M. Bhuyan A. et al.; Cell Physiol. Biochem. 40, 1129 (2016), Abstract; Full Text
Stimulation of Suicidal Erythrocyte Death by Rottlerin: M. Mischitelli, et al.; Cell. Physiol. Biochem. 40, 558 (2016), Abstract;
Stimulation of Suicidal Erythrocyte Death by the CDC25 Inhibitor NSC-95397: M. Jemaa, et al.; Cell. Physiol. Biochem. 40, 597 (2016), Abstract;
Targeting acid sphingomyelinase with anti-angiogenic chemotherapy: J. Jacobi, et al.; Cell Signal. 29, 52 (2016), Application(s): CRM detection by confocal microscopy, Abstract;
Triggering of Erythrocyte Cell Membrane Scrambling by Emodin: M. Mischitelli, et al.; Cell. Physiol. Biochem. 40, 91 (2016), Abstract;
Triggering of Suicidal Erythrocyte Death by Bexarotene: A.M. Bhuyan A. et al.; Cell Physiol. Biochem. 40, 1239 (2016), Abstract; Full Text
Triggering of Suicidal Erythrocyte Death by Pazopanib: E. Signoretto, et al.; Cell Physiol. Biochem. 38, 926 (2016), Application(s): Monoclonal antibody-based assay, Abstract; Full Text
C6-pyridinium ceramide sensitizes SCC17B human head and neck squamous cell carcinoma cells to photodynamic therapy: N.B. Boppana, et al.; J. Photochem. Photobiol. B 143, 163 (2015), Application(s): Immunocytochemistry using human head and neck squamous cell carcinoma cells SCC17B, Abstract;
Enhanced killing of SCC17B human head and neck squamous cell carcinoma cells after photodynamic therapy plus fenretinide via the de novo sphingolipid biosynthesis pathway and apoptosis: N.B. Boppana, et al.; Int. J. Oncol. 46, 2003 (2015), Abstract; Full Text
Enhanced Suicidal Erythrocyte Death Contributing to Anemia in the Elderly: A. Lupescu, et al.; Cell. Physiol. Biochem. 36, 773 (2015), Application(s): Assay using human erythrocytes, Abstract; Full Text
Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity: B. Jaishy, et al.; J. Lipid Res. 56, 546 (2015), Application(s): Immunocytochemistry using rat cardiomyocytes, Abstract; Full Text
Stimulation of Eryptosis by Narasin: G. Bouguerra, et al.; Cell Physiol Biochem. 37, 1807 (2015), Application(s): Cell staining, Abstract; Full Text
Ultrasound-stimulated microbubble enhancement of radiation treatments: endothelial cell function and mechanism: A.A. Al-Mahrouki, et al.; Oncoscience 2, 944 (2015), Abstract; Full Text
Defective macroautophagic turnover of brain lipids in the TgCRND8 Alzheimer mouse model: prevention by correcting lysosomal proteolytic deficits: D.S. Yang, et al.; Brain 137, 3300 (2014), Abstract; Full Text
Quantitative Ultrasound Characterization of Tumor Cell Death: Ultrasound-Stimulated Microbubbles for Radiation Enhancement: H.C. Kim, et al.; PLoS One 9, e102343 (2014), Application(s): Immunohistochemistry using formalin-fixed, paraffin-embedded tumor tissue samples from SCID mice bearing PC-3 xenografts, Abstract; Full Text
Effects of biophysical parameters in enhancing radiation responses of prostate tumors with ultrasound-stimulated microbubbles: H.C. Kim, et al.; Ultrasound Med. Biol. 39, 1376 (2013), Abstract;
Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury: S. Kim, et al.; Brain Pathol. 22, 41 (2012), Abstract;
Ceramide mediates nanovesicle shedding and cell death in response to phosphatidylinositol ether lipid analogs and perifosine: J.J. Gills, et al.; Cell Death Dis. 3, e340 (2012), Abstract; Full Text
Monitoring ceramide and sphingosine-1-phosphate levels in cancer cels and macrophages from tumours treated by photodynamic therapy: M. Korbelik, et al.; Photochem. Photobiol. Sci. 11, 779 (2012), Abstract;
Inhibition of SREBP1 sensitizes cells to death ligands: Y. Eberhard, et al.; Oncotarget 2, 186 (2011), Abstract; Full Text
MDR1 (multidrug resistence 1) can regulate GCS (glucosylceramide synthase) in breast cancer cells: X. Zhang, et al.; J. Surg. Oncol. 104, 466 (2011), Abstract;
Triggering of erythrocyte cell membrane scrambling by ursolic acid: K. Jilani, et al.; J. Nat. Prod. 74, 2181 (2011), Abstract;
Induction of membrane ceramides: a novel strategy to interfere with T lymphocyte cytoskeletal reorganisation in viral immunosuppression: E. Gassert, et al.; PLoS Pathog. 5, e1000623 (2009), Abstract; Full Text
Caspase-dependent and -independent activation of acid sphingomyelinase signaling: J.A. Rotolo, et al.; J. Biol. Chem. 280, 26425 (2005), Abstract; Full Text
Inhibition of erythrocyte phosphatidylserine exposure by urea and Cl: K.S. Lang, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 286, F1046 (2004), Abstract;
Involvement of ceramide in hyperosmotic shock-induced death of erythrocytes: K.S. Lang, et al.; Cell Death Differ. 11, 231 (2004), Abstract;
PAF-mediated pulmonary edema: a new role for acid sphingomyelinase and ceramide: R. Goggel, et al.; Nat. Med 10, 155 (2004), Abstract;
Study on ceramide expression and DNA content in patients with healthy mucosa, leukoplakia, and carcinoma of the larynx: F.L. Chi, et al.; Arch. Otolaryngol. Head Neck Surg. 130, 307 (2004), Abstract;
Ceramide inhibits the potassium channel Kv1.3 by the formation of membrane platforms: J. Bock, et al.; BBRC 305, 890 (2003), Abstract;
Host defense against Pseudomonas aeruginosa requires ceramide-rich membrane rafts: H. Grassme, et al.; Nat. Med. 9, 322 (2003), Abstract;
Regulation of cell death in mitotic neural progenitor cells by asymmetric distribution of prostate apoptosis response 4 (PAR-4) and simultaneous elevation of endogenous ceramide: E. Bieberich, et al.; J. Cell Biol. 162, 469 (2003), Abstract; Full Text
Ceramide-Rich Membrane Rafts Mediate CD40 Clustering: H. Grassme, et al.; J. Immunol. 168, 298 (2002), Abstract; Full Text
Clustering of CD40 ligand is required to form a functional contact with CD40: H. Grassme, et al.; J. Biol. Chem. 277, 30289 (2002), Abstract;
Structural determinants of sphingolipid recognition by commercially available anti-ceramide antibodies: L.A. Cowart, et al.; J. Lipid Res. 43, 2042 (2002), Abstract; Full Text
CD95 Signaling Via Ceramide-rich Membrane Rafts: H. Grassme, et al.; J. Biol. Chem. 276, 20589 (2001), Abstract; Full Text

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