Product Details
| Clone: | MID 15B4 |
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| Host: | Mouse |
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| Isotype: | IgM |
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| Immunogen: | Ceramide (sphingosine-[trans-D-erythro-2-amino-4-octadecene-1,3-diol]) conjugated to BSA. |
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| Source: | Purified from ascites by gel filtration on sephacryl S-300. |
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| Species reactivity: | Species independent
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| Specificity: | Recognizes C16- and C24-ceramide, dihydroceramide, sphingomyelin and phosphatidylcholine in highly artificial lipid overlay test systems. Under more physiological in vitro and in vivo conditions highly specific for ceramide and does not cross-react with sphingomyelin, cholesterol or other phospholipids. |
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| Applications: | ELISA, Flow Cytometry, ICC, IHC (PS)
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| Recommended Dilutions/Conditions: | ELISA (1:10)
Immunohistochemistry (1:10)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Quantity: | 1ml (50 tests). Suggested amount: 20µl/test. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 0.5M sodium chloride, 0.1% BSA and 0.09% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Technical Info/Product Notes: | Cited samples:
For an overview on cited samples please click here. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Sphingomyelinase modulates synaptic vesicle mobilization at the mice neuromuscular junctions: A.N. Tsentsevitsky, et al.; Life Sci.
318, 121507 (2023),
Abstract;
Dependence of ABCB1 transporter expression and function on distinct sphingolipids generated by ceramide synthases-2 and -6 in chemoresistant renal cancer: W.K. Lee, et al.; Int. J. Mol. Med.
31, 101492 (2022),
Abstract;
Acid sphingomyelinase promotes SGK1-dependent vascular calcification: T. Luong, et al.; Clin. Sci.
135, 515 (2021),
Abstract;
Full Text
Probing Physical Properties of the Cellular Membrane in Senescent Cells by Fluorescence Imaging: J.H. Wi, et al.; J. Phys. Chem. B
125, 10182 (2021),
Abstract;
S100B/RAGE/Ceramide signaling pathway is involved in sepsis-associated encephalopathy: L. Zhang, et al.; Life Sci.
277, 119490 (2021),
Abstract;
Medial calcification in the arterial wall of smooth muscle cell‐specific Smpd1 transgenic mice: A ceramide‐mediated vasculopathy: O.M. Bhat, et al.; J. Cell. Mol. Med.
24, 539 (2020),
Abstract;
Full Text
Apurinic/apyrimidinic endonuclease/redox factor 1 (APE1) alleviates myocardial hypoxia-reoxygenation injury by inhibiting oxidative stress and ameliorating mitochondrial dysfunction: J. Hao, et al.; Exp. Ther. Med.
17, 2143 (2019),
Abstract;
Full Text
Autophagy augmentation alleviates cigarette smoke-induced CFTR-dysfunction, ceramide-accumulation and COPD-emphysema pathogenesis: M. Bodas, et al.; Free Radic. Biol. Med.
131, 81 (2019),
Application(s): Co-staining and fluorescence microscopy using human and murine lung tissue sections,
Abstract;
Comparison of ceramide retention in the stratum corneum between dry skin and normal skin using animal model with fluorescent imaging method: M. Aoki, et al.; Skin Res. Technol.
25, 158 (2019),
Abstract;
Mechanism of palmitic acid-induced deterioration of in vitro development of porcine oocytes and granulosa cells: H. Shibahara, et al.; Theriogenology
141, 54 (2019),
Abstract;
Novel sphingomyelin biomarkers for brain glioma and associated regulation research on the PI3K/Akt signaling pathway: X.H. Zhai, et al.; Oncol. Lett.
18, 6207 (2019),
Abstract;
Full Text
Rab25 Deficiency Perturbs Epidermal Differentiation and Skin Barrier Function in Mice: H. Jeong, et al.; Biomol. Ther. (Seoul)
27, 553 (2019),
Abstract;
Full Text
Heterotrimeric G-protein subunit Gαi2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets: H. Cao, et al.; Physiol. Rep.
6, 13841 (2018),
Abstract;
Full Text
Nuclear phosphatidylinositol 4,5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription: M. Sobol, et al.; J. Cell Sci.
131, jcs211094 (2018),
Abstract;
Palmitic acid induces ceramide accumulation, mitochondrial protein hyper-acetylation and mitochondrial dysfunction in porcine oocytes: N. Itami, et al.; Biol. Reprod.
98, 644 (2018),
Abstract;
Pyridostigmine protects against cardiomyopathy associated with adipose tissue browning and improvement of vagal activity in high-fat diet rats: Y. Lu, et al.; Biochim. Biophys. Acta
1864, 1037 (2018),
Abstract;
Stress-induced host membrane remodeling protects from infection by non-motile bacterial pathogens: C. Tawk, et al.; EMBO J.
37, e98529 (2018),
Abstract;
Full Text
Defective Sphingosine-1-phosphate metabolism is a druggable target in Huntington's disease: A. Di Pardo, et al.; Sci. Rep.
7, 5280 (2017),
Abstract;
Full Text
Inhibition of Suicidal Erythrocyte Death by Volasertib: A. Al Mamun Bhuyan, et al.; Cell. Physiol. Biochem.
43, 1 (2017),
Abstract;
Full Text
Wash or wipe? A comparative study of skin physiological changes between water washing and wiping after skin cleaning: K. Ogai, et al.; Skin Res. Technol.
23, 519 (2017),
Abstract;
Anidulafungin-Induced Suicidal Erythrocyte Death: T. Peter, et al.; Cell. Physiol. Biochem.
38, 2272 (2016),
Application(s): Flow cytometry,
Abstract;
Full Text
Bile Acid-Induced Suicidal Erythrocyte Death: E. Lang, et al.; Cell Physiol. Biochem.
38, 1500 (2016),
Application(s): Cell culture,
Abstract;
Full Text
Enhanced Eryptosis Following Exposure to Dolutegravir: A. Al Mamun Bhuyan, et al.; Cell Physiol. Biochem.
39, 639 (2016),
Application(s): Ceramide abundance detection, by flow cytometric analysis,
Abstract;
Full Text
Estrogen Metabolite 16α-Hydroxyestrone Exacerbates Bone Morphogenetic Protein Receptor Type II-Associated Pulmonary Arterial Hypertension Through MicroRNA-29-Mediated Modulation of Cellular Metabolism: X. Chen, et al.; Circulation
133, 82 (2016),
Application(s): Immunostaining,
Abstract;
Full Text
Galectin-1 is a local but not systemic immunomodulatory factor in mesenchymal stromal cells: R. Fajka-Boja, et al.; Cytotherapy
18, 360 (2016),
Application(s): Cell staining,
Abstract;
Micafungin-Induced Suicidal Erythrocyte Death: T. Peter, et al.; Cell Physiol. Biochem.
39, 584 (2016),
Application(s): Determine ceramide abundance at the erythrocyte surface,
Abstract;
Full Text
Nocodazole Induced Suicidal Death of Human Erythrocytes: E. Signoretto, et al.; Cell. Physiol. Biochem.
38, 379 (2016),
Application(s): Cell staining,
Abstract;
Full Text
oxLDL and eLDL Induced Membrane Microdomains in Human Macrophages: S. Wallner, et al.; PLoS One
11, e0166798 (2016),
Abstract;
Full Text
Stimulated Suicidal Erythrocyte Death in Arteritis: R. Bissinger, et al.; Cell. Physiol. Biochem.
39, 1068 (2016),
Application(s): Determination of ceramide formation, erythrocytes,
Abstract;
Full Text
Stimulating Effect of Sclareol on Suicidal Death of Human Erythrocytes: E. Signoretto, et al.; Cell Physiol. Biochem.
39, 554 (2016),
Application(s): Determination of Ceramide abundance,
Abstract;
Full Text
Stimulation of Suicidal Erythrocyte Death by Ceritinib-Treatment of Human Erythrocytes: A.M. Bhuyan A. et al.; Cell Physiol. Biochem.
40, 1129 (2016),
Abstract;
Full Text
Stimulation of Suicidal Erythrocyte Death by Rottlerin: M. Mischitelli, et al.; Cell. Physiol. Biochem.
40, 558 (2016),
Abstract;
Stimulation of Suicidal Erythrocyte Death by the CDC25 Inhibitor NSC-95397: M. Jemaa, et al.; Cell. Physiol. Biochem.
40, 597 (2016),
Abstract;
Targeting acid sphingomyelinase with anti-angiogenic chemotherapy: J. Jacobi, et al.; Cell Signal.
29, 52 (2016),
Application(s): CRM detection by confocal microscopy,
Abstract;
Triggering of Erythrocyte Cell Membrane Scrambling by Emodin: M. Mischitelli, et al.; Cell. Physiol. Biochem.
40, 91 (2016),
Abstract;
Triggering of Suicidal Erythrocyte Death by Bexarotene: A.M. Bhuyan A. et al.; Cell Physiol. Biochem.
40, 1239 (2016),
Abstract;
Full Text
Triggering of Suicidal Erythrocyte Death by Pazopanib: E. Signoretto, et al.; Cell Physiol. Biochem.
38, 926 (2016),
Application(s): Monoclonal antibody-based assay,
Abstract;
Full Text
C6-pyridinium ceramide sensitizes SCC17B human head and neck squamous cell carcinoma cells to photodynamic therapy: N.B. Boppana, et al.; J. Photochem. Photobiol. B
143, 163 (2015),
Application(s): Immunocytochemistry using human head and neck squamous cell carcinoma cells SCC17B,
Abstract;
Enhanced killing of SCC17B human head and neck squamous cell carcinoma cells after photodynamic therapy plus fenretinide via the de novo sphingolipid biosynthesis pathway and apoptosis: N.B. Boppana, et al.; Int. J. Oncol.
46, 2003 (2015),
Abstract;
Full Text
Enhanced Suicidal Erythrocyte Death Contributing to Anemia in the Elderly: A. Lupescu, et al.; Cell. Physiol. Biochem.
36, 773 (2015),
Application(s): Assay using human erythrocytes,
Abstract;
Full Text
Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity: B. Jaishy, et al.; J. Lipid Res.
56, 546 (2015),
Application(s): Immunocytochemistry using rat cardiomyocytes,
Abstract;
Full Text
Stimulation of Eryptosis by Narasin: G. Bouguerra, et al.; Cell Physiol Biochem.
37, 1807 (2015),
Application(s): Cell staining,
Abstract;
Full Text
Ultrasound-stimulated microbubble enhancement of radiation treatments: endothelial cell function and mechanism: A.A. Al-Mahrouki, et al.; Oncoscience
2, 944 (2015),
Abstract;
Full Text
Defective macroautophagic turnover of brain lipids in the TgCRND8 Alzheimer mouse model: prevention by correcting lysosomal proteolytic deficits: D.S. Yang, et al.; Brain
137, 3300 (2014),
Abstract;
Full Text
Quantitative Ultrasound Characterization of Tumor Cell Death: Ultrasound-Stimulated Microbubbles for Radiation Enhancement: H.C. Kim, et al.; PLoS One
9, e102343 (2014),
Application(s): Immunohistochemistry using formalin-fixed, paraffin-embedded tumor tissue samples from SCID mice bearing PC-3 xenografts,
Abstract;
Full Text
Effects of biophysical parameters in enhancing radiation responses of prostate tumors with ultrasound-stimulated microbubbles: H.C. Kim, et al.; Ultrasound Med. Biol.
39, 1376 (2013),
Abstract;
Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury: S. Kim, et al.; Brain Pathol.
22, 41 (2012),
Abstract;
Ceramide mediates nanovesicle shedding and cell death in response to phosphatidylinositol ether lipid analogs and perifosine: J.J. Gills, et al.; Cell Death Dis.
3, e340 (2012),
Abstract;
Full Text
Monitoring ceramide and sphingosine-1-phosphate levels in cancer cels and macrophages from tumours treated by photodynamic therapy: M. Korbelik, et al.; Photochem. Photobiol. Sci.
11, 779 (2012),
Abstract;
Inhibition of SREBP1 sensitizes cells to death ligands: Y. Eberhard, et al.; Oncotarget
2, 186 (2011),
Abstract;
Full Text
MDR1 (multidrug resistence 1) can regulate GCS (glucosylceramide synthase) in breast cancer cells: X. Zhang, et al.; J. Surg. Oncol.
104, 466 (2011),
Abstract;
Triggering of erythrocyte cell membrane scrambling by ursolic acid: K. Jilani, et al.; J. Nat. Prod.
74, 2181 (2011),
Abstract;
Induction of membrane ceramides: a novel strategy to interfere with T lymphocyte cytoskeletal reorganisation in viral immunosuppression: E. Gassert, et al.; PLoS Pathog.
5, e1000623 (2009),
Abstract;
Full Text
Caspase-dependent and -independent activation of acid sphingomyelinase signaling: J.A. Rotolo, et al.; J. Biol. Chem.
280, 26425 (2005),
Abstract;
Full Text
Inhibition of erythrocyte phosphatidylserine exposure by urea and Cl: K.S. Lang, et al.; Am. J. Physiol. Lung Cell Mol. Physiol.
286, F1046 (2004),
Abstract;
Involvement of ceramide in hyperosmotic shock-induced death of erythrocytes: K.S. Lang, et al.; Cell Death Differ.
11, 231 (2004),
Abstract;
PAF-mediated pulmonary edema: a new role for acid sphingomyelinase and ceramide: R. Goggel, et al.; Nat. Med
10, 155 (2004),
Abstract;
Study on ceramide expression and DNA content in patients with healthy mucosa, leukoplakia, and carcinoma of the larynx: F.L. Chi, et al.; Arch. Otolaryngol. Head Neck Surg.
130, 307 (2004),
Abstract;
Ceramide inhibits the potassium channel Kv1.3 by the formation of membrane platforms: J. Bock, et al.; BBRC
305, 890 (2003),
Abstract;
Host defense against Pseudomonas aeruginosa requires ceramide-rich membrane rafts: H. Grassme, et al.; Nat. Med.
9, 322 (2003),
Abstract;
Regulation of cell death in mitotic neural progenitor cells by asymmetric distribution of prostate apoptosis response 4 (PAR-4) and simultaneous elevation of endogenous ceramide: E. Bieberich, et al.; J. Cell Biol.
162, 469 (2003),
Abstract;
Full Text
Ceramide-Rich Membrane Rafts Mediate CD40 Clustering: H. Grassme, et al.; J. Immunol.
168, 298 (2002),
Abstract;
Full Text
Clustering of CD40 ligand is required to form a functional contact with CD40: H. Grassme, et al.; J. Biol. Chem.
277, 30289 (2002),
Abstract;
Structural determinants of sphingolipid recognition by commercially available anti-ceramide antibodies: L.A. Cowart, et al.; J. Lipid Res.
43, 2042 (2002),
Abstract;
Full Text
CD95 Signaling Via Ceramide-rich Membrane Rafts: H. Grassme, et al.; J. Biol. Chem.
276, 20589 (2001),
Abstract;
Full Text
