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Phosphoserine monoclonal antibody (7F12)

ALX-804-166-C100 100 µg 710.00 USD
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Replaces Prod. #: BML-SA336

Product Details

Immunogen:Phosphoserine containing peptides conjugated to KLH.
Species reactivity:Species independent
Specificity:Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with phosphoserine surrounded by positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.). Please note that phosphoserine detection by MAbs always depends on the surrounding aa sequence.
Applications:ELISA, IP, WB
Recommended Dilutions/Conditions:Western Blot (1µg/ml HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS, 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Positive Control:Included. (Prod. No. 840-012-1)
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 1 week.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Shipping:Blue Ice
Long Term Storage:-20°C
Technical Info/Product Notes:Replacement for ADI-905-806
Regulatory Status:RUO - Research Use Only

Product Literature References

Phosphorylation of Bax Ser184 by Akt Regulates Its Activity and Apoptosis in Neutrophils: : S.J. Gardai, et al.; J. Biol. Chem. 279, 21085 (2004), Abstract;
Light regulation of protein phosphorylation in Blepharisma japonicum: H. Fabzak et al.; Acta Protozool. 40, 311 (2001),

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