Replaces Prod. #: BML-SA234
Product Details
Alternative Name: | CaM kinase II α subunit, CAMK2 α subunit |
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Clone: | 22B1 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to aa 281-294 (M281HRQET(PSO3)VDCLKKFN294) of the α-subunit of mouse and rat CaM kinase II (Ca2+/Calmodulin-dependent Protein Kinase II) thiophosphorylated at (Thr286). |
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UniProt ID: | P11798 (mouse), P11275 (rat) |
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Species reactivity: | Human, Mouse, Rat
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Specificity: | Recognizes the phosphorylated α-subunit of CaM kinase II. This antibody does not detect the non-phosphorylated α-subunit of rat CaM kinase II nor the ~60kDa β-subunit in either phosphorylation state. |
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Applications: | ELISA, Flow Cytometry, ICC, IF, IP, WB
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Recommended Dilutions/Conditions: | Flow Cytometry (FACS, 1μg 106 cells) Immunocytochemistry (1:100) Immunofluorescence (1:100) Western Blot (1:2,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Formulation: | Liquid. In PBS containing 0.05% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Cytoplasmic polyadenylation element binding protein-dependent protein synthesis is regulated by calcium/calmodulin-dependent protein kinase II: C.M. Atkins, et al.; J. Neurosci.
24, 5193 (2004),
Abstract;
Full Text
Targeted genomic disruption of H-ras and N-ras, individually or in combination, reveals the dispensability of both loci for mouse growth and development: L.M. Esteban, et al.; Mol. Cell. Biol.
21, 1444 (2001),
Abstract;
Full Text
Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- and dephosphokinase with complementary phosphorylation site-specific antibodies: B.L. Patton, et al.; Mol. Biol. Cell
4, 159 (1993),
Abstract;