Replaces Prod. #: ALX-801-016
Product Details
| Alternative Name: | Multidrug resistance-associated protein 2, cMOAT, ABCC2 |
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| Clone: | M2III-6 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant C-terminal rat MRP2 (multidrug resistance-associated protein-2; cMOAT) (aa 1339-1541). |
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| UniProt ID: | Q63120 |
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| Species reactivity: | Human, Rat
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| Specificity: | Recognizes an internal epitope of MRP2. |
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| Crossreactivity: | Does not cross-react with human P-glycoprotein, MDR3, MRP1, MRP3 or MRP5. |
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| Applications: | ICC, IHC (FS), IHC (PS), WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations.
Immunohistochemistry (frozen sections) (1:20): Can be use on acetone fixed frozen sections. Can be followed by incubation with rabbit anti-mouse antibody and a monoclonal mouse APAAP complex.
Immunohistochemistry (paraffin sections) (1:20, weak): Can be used on formaldehyde-fixed paraffin-embedded tissues and tumors, after pretreatment with 0.01M citric acid (pH 6.0) in distilled water at 100°C for 5 min 3 times. After incubation with the primary antibody and washing, slides can be incubated with biotinylated rabbit anti-mouse antibody and streptavidin conjugated to horseradish peroxidase.
Western Blot: Use 1:20-1:50 and anti-mouse HRP.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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| Formulation: | Liquid. In PBS containing 0.1% BSA and 0.02% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A microfluidic thyroid-liver platform to assess chemical safety in humans: J. Kühnlenz, et al.; ALTEX
40, 61 (2023),
Abstract;
Galectin-1 confers resistance to doxorubicin in hepatocellular carcinoma cells through modulation of P-glycoprotein expression: P. Carabias, et al.; Cell Death Discov.
13, 239 (2022),
Abstract;
Iron overload reduces synthesis and elimination of bile acids in rat liver: A. Prasnicka, et al.; Sci. Rep.
9, 9780 (2019),
Abstract;
Full Text
Mitogen-activated protein kinases are involved in hepatocanalicular dysfunction and cholestasis induced by oxidative stress: F.D. Toledo, et al.; Arch. Toxicol.
91, 2391 (2017),
Abstract;
Partial external biliary diversion in bile salt export pump deficiency: Association between outcome and mutation: P. Ellinger, et al.; World J. Gastroenterol.
23, 5295 (2017),
Application(s): Immunofluorescence staining of liver samples,
Abstract;
Full Text
Alteration of human hepatic drug transporter activity and expression by cigarette smoke condensate: K. Sayyed, et al.; Toxicology
363, 58 (2016),
Application(s): Cell culture; Effects on ABC transporter activities,
Abstract;
Analysis of the Bile Salt Export Pump (ABCB11) Interactome Employing Complementary Approaches: S. Przybylla, et al.; PLoS One
11, e0159778 (2016),
Application(s): Immunofluorescence staining of liver cryosections and differentiated HepaRG cells,
Abstract;
Full Text
Exon-skipping and mRNA decay in human liver tissue: molecular consequences of pathogenic bile salt export pump mutations: C. Droge, et al.; Sci. Rep.
6, 24827 (2016),
Application(s): Immunofluorescence of liver tissue,
Abstract;
The antitripanocide benznidazole promotes adaptive response to oxidative injury: Involvement of the nuclear factor-erythroid 2-related factor-2 (Nrf2) and multidrug resistance associated protein 2 (MRP2): J.P. Rigalli, et al.; Toxicol. Appl. Pharmacol.
304, 90 (2016),
Application(s): Western blot,
Abstract;
Anti-apoptotic effect of modified Chunsimyeolda-tang, a traditional Korean herbal formula, on MPTP-induced neuronal cell death in a Parkinson's disease mouse model: H. Li, et al.; J. Ethnopharmacol.
176, 336 (2015),
Application(s): Western blot,
Abstract;
Coordinated induction of GST and MRP2 by cAMP in Caco-2 cells: Role of protein kinase A signaling pathway and toxicological relevance: M.R. Arana, et al.; Toxicol. Appl. Pharmacol.
287, 178 (2015),
Application(s): Cell culture ,
Abstract;
Evaluation of transport of common antiepileptic drugs by human multidrug resistance-associated proteins (MRP1, 2 and 5) that are overexpressed in pharmacoresistant epilepsy: C. Luna-Tortos, et al.; Neuropharmacology
58, 1019 (2010),
Abstract;
Multiple pathways for fluoroquinolone secretion by human intestinal epithelial (Caco-2) cells: S. Lowes, et al.; Br. J. Pharmacol.
135, 1263 (2002),
Abstract;
Full Text
Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res.
60, 5269 (2000),
Abstract;
Full Text
Antifolate resistance mediated by the multidrug resistance proteins MRP1 and MRP2: J.H. Hooijberg, et al.; Cancer Res.
59, 2532 (1999),
Abstract;
Full Text
Drug export activity of the human canalicular multispecific organic anion transporter in polarized kidney MDCK cells expressing cMOAT (MRP2) cDNA: R. Evers, et al.; J. Clin. Invest.
101, 1310 (1998),
Abstract;
Full Text
Congenital jaundice in rats with a mutation in a multidrug resistance-associated protein gene: C.C. Paulusma, et al.; Science
271, 1126 (1996),
Abstract;
General Literature References
Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines : M. Kool, et al.; Cancer Res.
57, 3537 (1997),
Abstract;
