Product Details
Alternative Name: | Multidrug resistance-associated protein 2, cMOAT, ABCC2 |
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Clone: | M2I-4 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Recombinant human MRP2 (multidrug resistance-associated protein 2; cMOAT) (aa 215-310). |
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UniProt ID: | Q92887 |
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Species reactivity: | Human
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Specificity: | Recognizes an internal epitope of human MRP2. |
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Crossreactivity: | Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP3 or MRP5. Does not cross-react with mouse, rat or guinea pig MRP2. |
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Applications: | ICC, IHC (FS), WB
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Recommended Dilutions/Conditions: | Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations. Immunohistochemistry (frozen sections): Use 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex. Or, after incubation with the primary antibody and washing, slides can be incubated with biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase. Western Blot: Use 1:20-1:50 dilution and anti-mouse-HRP. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Immunohistochemistry: Suitable for frozen sections, weak in paraffin sections. |
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Formulation: | Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | +4°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Structural analogues of roscovitine rescue the intracellular traffic and the function of ER-retained ABCB4 variants in cell models: V. Vauthier, et al.; Sci. Rep.
9, 6653 (2019),
Abstract;
Full Text
Functional Defect of Variants in the ATP-binding Sites of ABCB4 and their Rescue by the CFTR Potentiator, Ivacaftor (VX-770): J. Delaunay, et al.; Hepatology
65, 560 (2017),
Abstract;
Partial external biliary diversion in bile salt export pump deficiency: Association between outcome and mutation: P. Ellinger, et al.; World J. Gastroenterol.
23, 5295 (2017),
Application(s): Immunofluorescence staining of liver samples,
Abstract;
Full Text
Exon-skipping and mRNA decay in human liver tissue: molecular consequences of pathogenic bile salt export pump mutations: C. Droge, et al.; Sci. Rep.
6, 24827 (2016),
Application(s): Immunofluorescence of liver tissue,
Abstract;
Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res.
60, 5269 (2000),
Abstract;
Full Text
General Literature References
Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines: M. Kool, et al.; Cancer Res.
57, 3537 (1997),
Abstract;
Congenital jaundice in rats with a mutation in a multidrug resistance-associated protein gene: C.C. Paulusma, et al.; Science
271, 1126 (1996),
Abstract;