Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 


Fluorescent probe for Ca2+. Cell permeable.
ALX-620-003-M001 1 mg 282.00 USD
Do you need bulk/larger quantities?
Cell permeable analog of FLUO 3. Fluorescent probe for Ca2+. After crossing the membrane, the product is quickly metabolized by cytoplasmic esterases to the membrane impermeant FLUO 3.

Product Details

Alternative Name:1-[Amino-5-(2,7-dichloro-6-acetomethoxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxy-methyl ester
Purity:≥85% (HPLC)
Identity:Determined by 1H-NMR and fluorescence.
Appearance:Red solid.
Solubility:Soluble in DMSO (0.9mg/ml) and acetonitrile (2mg/ml).
Long Term Storage:-20°C
Handling:Protect from light.
Protocol:General Protocol (for Human T cells)

2mM Fluo 3/AM/DMSO (1mg Fluo 3/AM in 442μl DMSO)
Pluronic F127
Hanks-balanced salt solution (HBSS)
HEPES buffer saline (10mM HEPES, 1mM Na2HPO4, 137mM NaCl, 5mM KCl, 1mM CaCl2, 0.5mM MgCl2, 5mM glucose, 0.1% BSA, pH 7.4)


  1. Add 16.5mg Pluronic F127 to Fluo 3/AM/DMSO solution. Pluronic F127 prevents aggregation of Fluo 3/AM in HBSS and helps uptake with cells.
  2. Dilute the Fluo 3/AM solution with HBSS to prepare 4μM Fluo 3/AM working solution.
  3. Add the Fluo 3/AM working solution to the cells, and incubate at 37°C for 20min.
  4. Add 5 volumes of HBSS containing 1% fetal calf serum, and continue the incubation for another 40min.
  5. Wash the cells 3 times with HEPES buffer saline. Then resuspend the cells to prepare 1x105 cells/ml solution using HEPES buffer saline.
  6. Incubate at 37°C for 10min. Then use the cells for fluorescent calcium ion detection.
  7. Monitor the fluorescence at 528nm (excitation: 490-500nm)

Cell staining conditions differ by cell types, so it is necessary to optimize the conditions for each experiment.

Regulatory Status:RUO - Research Use Only
Please mouse over

Product Literature References

The Us3 Protein of Herpes Simplex Virus 1 Inhibits T Cell Signaling by Confining LAT Activation via TRAF6: Y. Yang, et al.; J. Biol. Chem. 290, 15670 (2015), Application(s): Cell Culture, Abstract; Full Text
Recombinant human tumor necrosis factor alpha induces calcium oscillation and calcium-activated chloride current in human neutrophils. The role of calcium/calmodulin-dependent protein kinase: M.A. Schumann, et al.; J. Biol. Chem. 268, 2134 (1993), Abstract; Full Text
Control of mitochondrial matrix calcium: studies using fluo-3 as a fluorescent calcium indicator: A. Saavedra-Molina, et al.; BBRC 167, 148 (1990), Abstract;
Flow cytometric measurement of cytoplasmic free calcium in human peripheral blood T lymphocytes with fluo-3, a new fluorescent calcium indicator: P.A. Vadenberghe & J.L. Ceuppens; J. Immunol. Meth. 127, 197 (1990), Abstract;
Glutamate induces calcium waves in cultured astrocytes: long-range glial signaling: A.H. Cornell-Bell, et al.; Science 247, 470 (1990), Abstract;
Photochemically generated cytosolic calcium pulses and their detection by fluo-3: J.P.Y. Kao, et al.; J. Biol. Chem. 264, 8179 (1989), Abstract; Full Text

Related Literature

Product Flyers
Detect Activity of Major ABC Transporter Proteins
Detect Activity of Major ABC Transporter Proteins
Download as PDF

All new literature pieces

Recommend this page

Keep in touch

©2021 Enzo Life Sciences, Inc.,