Product Details
| Alternative Name: | TWEAK-R, TNFRSF 12A, Fibroblast growth factor-inducible immediate-early response protein 14, CD266 |
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| MW: | ~34kDa (SDS-PAGE). |
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| Source: | Produced in HEK 293 cells. The extracellular domain of mouse Fn14 (aa 1-75) is fused to the Fc portion of human IgG1. |
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| UniProt ID: | Q9CR75 |
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| Gene/Protein Identifier: | A003364 (UCSD Signaling Gateway ID) |
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| Concentration: | 1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; BioWhittaker). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds human and mouse TWEAK. |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to TWEAK. |
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| Reconstitution: | Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | The fibroblast growth factor-inducible-14 (Fn14) is a 129 aa type Ia transmembrane protein. Fn14 is a growth-factor regulated, immediate-early response protein. It is expressed in a developmental and adult tissue-specific manner. Fn14 expression decreases cellular adhesion to extracellular matrix proteins fibronectin and vitronectin and reduces cell growth and migration. It is expressed at high levels in liver cancer cells, in hepatocellular carcinoma specimens as well as in regenerating liver cells showing Fn14 may play a role in hepatocyte growth and liver neoplasia. Fn14 has been described to bind TWEAK (Apo-3 Ligand), a member of the TNF family. Historical data has shown that Fn14 inhibits TWEAK-mediated killing of Kym-1 target cells. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol.
16, 467 (2004),
Abstract;
Potential role of APRIL as autocrine growth factor for megakaryocytopoiesis: D. Bonci, et al.; Blood
104, 3169 (2004),
Abstract;
TWEAK mediates signal transduction and differentiation of RAW264.7 cells in the absence of Fn14/TweakR. Evidence for a second TWEAK receptor: T.C. Polek, et al.; J. Biol. Chem.
278, 32317 (2003),
Abstract;
Full Text
General Literature References
The molecular architecture of the TNF superfamily: J.L. Bodmer, et al.; TIBS
27, 19 (2002), (Review),
Abstract;
A novel TNF receptor family member binds TWEAK and is implicated in angiogenesis: S.R. Wiley, et al.; Immunity
15, 837 (2001),
Abstract;
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