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TNF-α (soluble) (mouse), (recombinant)

ALX-522-009-C050 50 µg 425.00 USD
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Product Details

Alternative Name:Tumor necrosis factor-α, TNFSF 2
MW:~20kDa (SDS-PAGE).
Source:Produced in E. coli. The extracellular domain of mouse TNF-α (tumor necrosis factor-α) (aa 77-235) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
UniProt ID:P06804
Gene/Protein Identifier:A002291 (UCSD Signaling Gateway ID)
Concentration:1mg/ml after reconstitution.
Formulation:Lyophilized. Contains PBS.
Purity:≥90% (SDS-PAGE)
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
Species reactivity:Human, Mouse, Rat
Specificity:Binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-061), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.
Application Notes:ELISA: binds to human TNF-R1 receptor.
Reconstitution:Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Shipping:Blue Ice
Long Term Storage:-20°C
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Technical Info/Product Notes:Historical lots have shown that it exerts its biological activity in a concentration range of 0.5-1ng/ml (WEHI 164 cells). 

FLAG is a registered trademark of Sigma-Aldrich Co.
Regulatory Status:RUO - Research Use Only
TNF-&alpha; (soluble) (mouse), (recombinant) SDS-PAGE
SDS-PAGE Analysis of TNF-α (soluble) (mouse), (recombinant). Lane 1: MW Marker, Lane 2: 1 µg TNF-α
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TNF-&alpha; (soluble) (mouse), (recombinant) SDS-PAGE

Product Literature References

RNPS1 inhibits excessive tumor necrosis factor/tumor necrosis factor receptor signaling to support hematopoiesis in mice: X. Zhong, et al.; PNAS 119, e2200128119 (2022), Abstract;
SMYD2 targets RIPK1 and restricts TNF-induced apoptosis and necroptosis to support colon tumor growth: Y.Q. Yu, et al.; Cell Death Dis. 13, 52 (2022), Abstract;
Simultaneous Inhibition of Three Major Cytokines and Its Therapeutic Effects: A Peptide-Based Novel Therapy against Endotoxemia in Mice: H.J. Shih, et al.; J. Pers. Med. 11, 436 (2021), Abstract;
K63-linked ubiquitination regulates RIPK1 kinase activity to prevent cell death during embryogenesis and inflammation: Y. Tang, et al.; Nat. Commun. 10, 4157 (2019), Abstract;
RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation: G. Liccardi, et al.; Mol. Cell 73, 413 (2019), Abstract; Full Text
Ubiquitination of RIPK1 suppresses programmed cell death by regulating RIPK1 kinase activation during embryogenesis: X. Zhang, et al.; Nat. Commun. 10, 4158 (2019), Abstract; Full Text
Ubiquitin-Mediated Regulation of RIPK1 Kinase Activity Independent of IKK and MK2: A. Annibaldi, et al.; Mol. Cell 69, 566 (2018), Abstract; Full Text
MK2 Phosphorylates RIPK1 to Prevent TNF-Induced Cell Death: I. Jaco, et al.; Mol. Cell 66, 698 (2017), Abstract; Full Text
A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood 117, 5953 (2011), Application(s): Death assay on mouse neutrophils, Abstract; Full Text
RasGAP-derived fragment N increases the resistance of beta cells towards apoptosis in NOD mice and delays the progression from mild to overt diabetes: N. Bulat, et al.; PLoS One 6, e22609 (2011), Abstract; Full Text
Inhibition of interleukin 1 receptor/Toll-like receptor signaling through the alternatively spliced, short form of MyD88 is due to its failure to recruit IRAK-4: K. Burns, et al.; J. Exp. Med. 197, 263 (2003), Abstract; Full Text
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998), Abstract; Full Text

General Literature References

The transmembrane form of tumor necrosis factor is the prime activating ligand of the 80 kDa tumor necrosis factor receptor: M. Grell, et al.; Cell 83, 793 (1995), Abstract;
The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death: C.A. Smith, et al.; Cell 76, 959 (1994), Abstract;
Tumor necrosis factor: a pleiotropic cytokine and therapeutic target: K.J. Tracey & A. Cerami; Annu. Rev. Med. 45, 491 (1994), Abstract;

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