Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 


Caspase-8 substrate
ALX-260-118-M005 5 mg 256.00 USD
ALX-260-118-M010 10 mg 484.00 USD
Do you need bulk/larger quantities?
Fluorogenic substrate for caspase-8.

Product Details

Alternative Name:Caspase-8 substrate (fluorogenic)
Sequence:Ac-Leu-Glu-Thr-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin)
Purity:≥97% (HPLC)
Appearance:White to off-white powder.
Solubility:Soluble in DMSO.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Use/Stability:20mM stock solution in DMSO is stable at -20°C for 6-8 months. When taking out of the freezer wait until it warms up to room temperature, then open it.
Handling:Keep cool and dry.
Technical Info/Product Notes:AFC has an excitation maximum of 400nm and an emission maximum of 505nm.
Protocol:HEPES-Buffer (2x): 40 mM HEPES, pH 7.5, 20% glycerol, 4mM DTT. Dilute to 1x with sterile distilled water prior to use.
Substrate: Prepare 20 mM stock solution in DMSO.

- Induce apoptosis and prepare cell lysate or use recombinant caspase.
- Prepare reaction buffer: 10µl of substrate stock solution + 1ml 1x HEPES-Buffer for each reaction.
- Add an appropriate amount of cell lysate (50- 100 µl; should be titrated) or recombinant caspase to reaction buffer.
- Incubate for 1 hour at 37 °C.
- Measure with spectrofluorometer: 400 nm excitation wavelength, 505 nm emission wavelength.

Suggested controls:
Reaction mixture without substrate.
Reaction mixture with non-apoptotic cell lysate.
Reaction mixture with apoptotic cell lysate and caspase inhibitor.
Regulatory Status:RUO - Research Use Only

Product Literature References

CRISPR knock out of programmed cell death protein 1 enhances anti-tumor activity of cytotoxic T lymphocytes: Z. Zhao, et al. ; Oncotarget 9, 5208 (2018), Abstract; Full Text
Protective effect of 3-hydroxybutyrate against endoplasmic reticulum stress-associated vascular endothelial cell damage induced by low glucose exposure: E. Soejima, et al.; PLoS One 13, e0191147 (2018), Abstract; Full Text
Epidermal growth factor signaling protects from cholestatic liver injury and fibrosis: J. Svinka, et al.; J. Mol. Med. (Berl.) 95, 109 (2017), Application(s): Detection of active caspases, liver hepatocytes, Abstract; Full Text
Redox regulation of metabolic and signaling pathways by thioredoxin and GLUTAREDOXIn in nos-3 overexpressing hepatoblastoma cells: R. González, et al.; Redox Biol. 6, 122 (2015), Application(s): Cell Culture, Fluorescence, Abstract;

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
Caspase Substrate
Caspase-8 Substrate
By catalog section:


Technical Service
Customer Service