Replaces Prod. #: BML-P464
Fluorogenic substrate for caspase-2; similar to Ac-VDVAD-AMC but the AFC fluorophore has a greater Stokes’ shift upon cleavage. VDVAD has been found to be a preferred cleavage site for caspase-2 (Ich-1L). Increases in Ac-VDVAD-AFC cleavage correlated with losses in procaspase-2 (conversion to active caspase-2) in human neuroblastoma lines induced to apoptosis with C2-ceramide and NO.
Product Details
| Alternative Name: | Caspase-2 substrate (fluorogenic) |
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| Sequence: | Ac-Val-Asp-Val-Ala-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin) |
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| Formula: | C33H41F3N6O12 |
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| MW: | 770.7 |
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| Peptide Content: | 75-95% |
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| Purity: | ≥98% (HPLC) |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Handling: | Warm up to room temperature before opening. Keep cool and dry. |
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| Technical Info/Product Notes: | AFC has an excitation maximum of 400nm and an emission maximum of 505nm. |
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| Regulatory Status: | RUO - Research Use Only |
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General Literature References
Mechanism of nitric oxide-induced apoptosis in human neuroblastoma SH-SY5Y cells: R. Moriya, et al.; FEBS Lett.
484, 253 (2000),
Abstract;
Possible involvement of cytochrome c release and sequential activation of caspases in ceramide-induced apoptosis in SK-N-MC cells: A. Ito, et al.; Biochim. Biophys. Acta
1452, 263 (1999),
Abstract;
Substrate specificities of caspase family proteases: R.V. Talanian, et al.; J. Biol. Chem.
272, 9677 (1997),
Abstract;
