Replaces Prod. #: BML-P439
Fluorogenic substrate for caspase-6 (Km=30μM; Kcat/Km=168000 M-1sec-1) and related cysteine proteases. Sequence is based on lamin A site of cleavage.
Product Details
| Alternative Name: | Caspase-6 substrate (fluorogenic) |
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| Sequence: | Ac-Val-Glu-Ile-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin) |
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| Formula: | C32H40F3N5011 |
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| MW: | 727.7 |
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| Purity: | ≥98% (HPLC) |
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| Solubility: | Soluble in DMSO or dimethyl formamide. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Handling: | Protect from light. |
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| Technical Info/Product Notes: | AFC has an excitation maximum of 400nm and an emission maximum of 505nm. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Exogenous Introduction of Initiator and Executioner Caspases Results in Different Apoptotic Outcomes: F. Anson, et al.; JACS Au
1, 1240 (2021),
Abstract;
Identification of Allosteric Inhibitors against Active Caspase-6: A. Tubelvicuite-Aydin, et al.; Sci. Rep.
9, 5504 (2019),
Abstract;
Full Text
Organ specific alteration in caspase expression and STK3 proteolysis during the aging process: M. Lessard-Beaudoin, et al.; Neurobiol. Aging
47, 50 (2016),
Application(s): Caspase activity assays,
Abstract;
Full Text
General Literature References
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem.
272, 17907 (1997),
Abstract;
Substrate specificities of caspase family proteases: R.V. Talanian, et al.; J. Biol. Chem.
272, 9677 (1997),
Abstract;
The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32: S.M. Srinivasula, et al.; J. Biol. Chem.
271, 27099 (1996),
Abstract;
