Fluorogenic substrate for caspase-4. Similar to the fluorogenic substrate Ac-LEVD-AMC (Prod. No. ALX-260-083); the AFC fluorophore is more sensitive.
Product Details
| Alternative Name: | Caspase-4 substrate (fluorogenic) |
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| Sequence: | Ac-Leu-Glu-Val-Asp-AFC (AFC=7-Amido-4-trifluoromethylcoumarin) |
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| Formula: | C32H40F3N5O11 |
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| MW: | 727.7 |
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| Peptide Content: | 75-95%. |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in DMSO; dilute with distilled water or buffer; also soluble in phosphate buffer, pH 7.5-8.0. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Dissolve only as much as needed at one time because of the possibility of decomposition in solution. Store solution at -20°C. Protect from condensation: when taking the sample out of the container, wait until it warms up to room temperature, then open it. |
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| Handling: | Keep cool and dry. |
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| Technical Info/Product Notes: | AFC has an excitation maximum of 400nm and an emission maximum of 505nm. |
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| Regulatory Status: | RUO - Research Use Only |
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