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Boc-D(OMe)-FMK

Caspase inhibitor
 
ALX-260-071-M001 1 mg 282.00 USD
 
ALX-260-071-M005 5 mg 1,137.00 USD
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Irreversible, cell permeable broad-spectrum caspase inhibitor.

Product Specification

Alternative Name:Caspase family inhibitor (fluoromethylketone)
 
Sequence:Boc-Asp(OMe)-fluoromethylketone
 
Formula:C11H18FNO5
 
MW:263.3
 
CAS:187389-53-3
 
Purity:≥95% (TLC)
 
Appearance:White to off-white powder.
 
Solubility:Soluble in DMSO or dimethyl formamide.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stock solutions should be divided into aliquots and stored at -20°C. For long term storage keep aliquots at -70°C.
 
Handling:Keep cool and dry. After reconstitution, prepare aliquots and store at -80°C.
 
Technical Info/Product Notes:The carboxyl groups are OMe ester form to enhance cell permeability. If purified enzyme is used, esterase should be added for the hydrolysis of the ester groups.
 
Protocol:Prepare a 20 mM stock solution of the caspase inhibitor in DMSO. Add 1 µl of above stock solutions to 1 ml of culture medium containing cells to give 20 mM final concentration. DMSO concentration above of 0.2 % may cause cellular toxicity thus masking the effect of the ICE-protease inhibitor Boc-D(OMe)-FMK. For in vivo experiments extending for 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation by reaction with cysteine protease.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Mechanisms of cell death induced by arginase and asparaginase in precursor B-cell lymphoblasts: L.E. Metayer, et al.; Apoptosis 24, 145 (2019), Abstract; Full Text
The neuroprotective effect of pituitary adenylate cyclase-activating polypeptide on cerebellar granule cells is mediated through inhibition of the CED3-related cysteine protease caspase-3/CPP32: D. Vaudry, et. al.; PNAS 97, 13390 (2000), Abstract; Full Text
A DEVD-inhibited caspase other than CPP32 is involved in the commitment of cerebellar granule neurons to apoptosis induced by K+ deprivation: S.R. D'Mello, et al.; J. Neurochem. 70, 1809 (1998), Abstract;
Caspase inhibitor affords neuroprotection with delayed administration in a rat model of neonatal hypoxic-ischemic brain injury: Y. Cheng, et al.; J. Clin. Invest. 101, 1992 (1998), Abstract; Full Text
Caspase inhibitor BD-fmk distinguishes transforming growth factor β-induced apoptosis from growth inhibition: T.L. Brown, et al.; Cell Growth Differ. 9, 869 (1998), Abstract;
Different interleukin-1 beta converting enzyme (ICE) family protease requirements for the apoptotic death of T lymphocytes triggered by diverse stimuli: A. Sarin, et al.; J. Exp. Med. 184, 2445 (1996), Abstract;

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