Irreversible, cell permeable broad-spectrum caspase inhibitor.
Product Details
| Alternative Name: | Caspase family inhibitor (fluoromethylketone) |
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| Sequence: | Boc-Asp(OMe)-fluoromethylketone |
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| Formula: | C11H18FNO5 |
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| MW: | 263.3 |
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| CAS: | 187389-53-3 |
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| Purity: | ≥95% (TLC) |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in DMSO or dimethyl formamide. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stock solutions should be divided into aliquots and stored at -20°C. For long term storage keep aliquots at -70°C. |
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| Handling: | Keep cool and dry. After reconstitution, prepare aliquots and store at -80°C. |
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| Technical Info/Product Notes: | The carboxyl groups are OMe ester form to enhance cell permeability. If purified enzyme is used, esterase should be added for the hydrolysis of the ester groups. |
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| Protocol: | Prepare a 20 mM stock solution of the caspase inhibitor in DMSO. Add 1 µl of above stock solutions to 1 ml of culture medium containing cells to give 20 mM final concentration. DMSO concentration above of 0.2 % may cause cellular toxicity thus masking the effect of the ICE-protease inhibitor Boc-D(OMe)-FMK. For in vivo experiments extending for 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation by reaction with cysteine protease. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Clostridioides difficile toxin B alone and with pro-inflammatory cytokines induces apoptosis in enteric glial cells by activating three different signalling pathways mediated by caspases, calpains and cathepsin B: K. Fettucciari, et al.; Cell. Mol. Life Sci.
79, 442 (2022),
Abstract;
Mechanisms of cell death induced by arginase and asparaginase in precursor B-cell lymphoblasts: L.E. Metayer, et al.; Apoptosis
24, 145 (2019),
Abstract;
Full Text
The neuroprotective effect of pituitary adenylate cyclase-activating polypeptide on cerebellar granule cells is mediated through inhibition of the CED3-related cysteine protease caspase-3/CPP32: D. Vaudry, et. al.; PNAS
97, 13390 (2000),
Abstract;
Full Text
A DEVD-inhibited caspase other than CPP32 is involved in the commitment of cerebellar granule neurons to apoptosis induced by K+ deprivation: S.R. D'Mello, et al.; J. Neurochem.
70, 1809 (1998),
Abstract;
Caspase inhibitor affords neuroprotection with delayed administration in a rat model of neonatal hypoxic-ischemic brain injury: Y. Cheng, et al.; J. Clin. Invest.
101, 1992 (1998),
Abstract;
Full Text
Caspase inhibitor BD-fmk distinguishes transforming growth factor β-induced apoptosis from growth inhibition: T.L. Brown, et al.; Cell Growth Differ.
9, 869 (1998),
Abstract;
Different interleukin-1 beta converting enzyme (ICE) family protease requirements for the apoptotic death of T lymphocytes triggered by diverse stimuli: A. Sarin, et al.; J. Exp. Med.
184, 2445 (1996),
Abstract;
