| ALX-210-762-C200 | 200 µg | 471.00 USD |  |
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| Alternative Name: | C-C chemokine receptor 8, CD198 |
| Host: | Goat |
| Immunogen: | Synthetic peptide corresponding to aa 12-36 (V12TDYYYPDIFSSPCDAELIQTNGKL36) of the N-terminal extracellular domain of human CCR8 (C-C chemokine receptor 8). |
| UniProt ID: | P51685 |
| Species reactivity: | Human |
| Applications: | ELISA, Flow Cytometry, IHC, WB |
| Recommended Dilutions/Conditions: | ELISA (1:50,000) Flow Cytometry (2-6µl per 106 cells/50-100µl) Immunohistochemistry (1:125) Western blot (1:500) Optimal conditions must be determined individually for each application. |
| Purity Detail: | Epitope-affinity purified IgG. |
| Formulation: | Affinity purified antibody in 10mM KHPO4, 140mM NaCl, 1mg/ml BSA and 0.1% sodium azide. |
| Handling: | For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Protocol: | Immunohistochemistry on paraffin sections: - Fix paraffin sections in 10% formalin. - De-wax sections and block endogenous peroxide by incubating sections with 3% H2O2 for 15 min. at room temperature (RT). - Wash sections with PBS and incubate with 2% normal rabbit serum at room temperature to block non-specific binding sites. - Wash sections with PBS and incubate with primary antibody (anti-chemokine receptor) at recommended dilution for 60 min. at RT. - Wash slides and incubate at RT with biotinylated rabbit anti-goat IgG H+L (Vector Labs) at 1:200 dilution for 30 min. - Wash slides and incubate with HRP-Streptavidin (Vector Labs) 1:400 dilution for 30 min. - Wash slides and incubate with DAB for 5-7 min. at RT. - Wash slides with PBS and counter stain with hematoxylin. |
| Regulatory Status: | RUO - Research Use Only |
