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Estrogen receptor β polyclonal antibody

ALX-210-132-C100 100 µg 814.00 USD
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Product Details

Alternative Name:ERβ
Immunogen:Synthetic peptide corresponding to aa 512-530 (C512SSTEDSKN KESSQNLQSQ530) of C-terminal rat ERβ (estrogen receptor β).
UniProt ID:Q62986
Species reactivity:Human, Mouse, Rat
Crossreactivity:Does not cross-react with ERα.
Applications:ICC, IF, IHC (FS), IHC (PS), WB
Recommended Dilutions/Conditions:Immunofluorescence (1:100-1:1,000)
Immunocytochemistry (1:100-1:1,000)
Immunohistochemistry (frozen sections, 1:50)
Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~55kDa by Western blot.
Purity Detail:Epitope-affinity purified.
Formulation:Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
Estrogen receptor β polyclonal antibody Immunofluorescence
Figure 1. Immunofluorescent analysis using ER-beta polyclonal antibody (Prod. No. ALX-210-132) shows nuclear localization of ER-beta in MCF7 cells.
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Estrogen receptor β polyclonal antibody Immunofluorescence

Product Literature References

Generation and reproductive phenotypes of mice lacking estrogen receptor beta: J.H. Krege, et al.; PNAS 95, 15677 (1998), Abstract; Full Text
Transcription and translation of estrogen receptor-beta in the male reproductive tract of estrogen receptor-alpha knock-out and wild-type mice: C.S. Rosenfeld, et al.; Endocrinology 139, 2982 (1998), Abstract;
Distribution of estrogen-beta-like immunoreactivity in rat forebrain: X. Li, et al.; Neuroendocrinology 66, 63 (1997), Abstract;

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