Product Details
| Alternative Name: | Leukemia inhibitory factor |
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| MW: | ~20kDa |
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| Source: | Produced in E. coli. |
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| UniProt ID: | P09056 |
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| Quantity: | 107U |
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| Formulation: | Liquid. 0.2μm-filtered solution in PBS containing 0.5% BSA. Contains no preservatives. Further dilutions should be made with medium containing 1% BSA. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Purity Detail: | Purified by FPLC. |
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| Biological Activity: | Induces macrophage differentiation and proliferation of hematopoietic stem cells; maintains indifferentiated state of of embryonic stem (ES) cell cultures; inhibits proliferation of the mouse myeloid cell line M1. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for 6 months when stored at +4°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Maintenance of ES Cells: 1,000U/ml are recommended to maintain indifferentiated ES cells, R1 or E14 cells.
M1Growth Inhibition Assay:50units/ml are defined as the concentration of LIF that induces 50% of differentiated cells in tissue culture medium. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Osteogenic Effect of Inducible Nitric Oxide Synthase (iNOS)-Loaded Mineralized Nanoparticles on Embryonic Stem Cells: J.S. Lee, et al.; Cell. Physiol. Biochem.
51, 746 (2018),
Abstract;
Leukemia inhibitory factor reduces body fat mass in ovariectomized mice: J.O. Jansson, et al.; Eur. J. Endocrinol.
154, 349 (2006),
Abstract;
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells: C.C. Shih, et al.; Blood
95, 1957 (2000),
Abstract;
Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells: R.L. Williams, et al.; Nature
336, 684 (1988),
Abstract;
