Product Details
| MW: | ~45kDa |
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| Source: | Produced in CHO cells. |
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| UniProt ID: | P10810 |
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| Concentration: | 0.25mg/ml after reconstitution. |
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| Formulation: | Lyophilized from PBS, pH 7.2. |
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| Purity: | ≥90-95% (SDS-PAGE) |
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| Purity Detail: | His-tag-affinity purified. |
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| Endotoxin Content: | <0.00015EU/µg protein (LAL test; Charles River). |
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| Biological Activity: | Inhibits binding of LPS to CD14. Up to 5µg/ml mouse CD14 inhibit binding of FITC-LPS (0.5µg/ml) to mouse CD14+ CHO transfectants (FACS). |
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| Reconstitution: | Reconstitute with 40µl sterile, ultra-pure distilled water. This solution can then be diluted with PBS or other buffers. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | The reconstituted protein should be used immediately or stored in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% BSA). |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 ist the 50kDa protein. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Absence of TRIF signaling in lipopolysaccharide-stimulated murine mast cells: S. Keck, et al.; J. Immunol.
186, 5478 (2011),
Abstract;
Monocytes can phagocytose Gram-negative bacteria by a CD14-dependent mechanism: U. Grunwald, et al.; J. Immunol.
157, 4119 (1996),
Abstract;
