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CD14 (mouse), (recombinant) (His-tag)

 
ALX-201-134-C010 10 µg 485.00 USD
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Product Specification

MW:~45kDa
 
Source:Produced in CHO cells.
 
UniProt ID:P10810
 
Concentration:0.25mg/ml after reconstitution.
 
Formulation:Lyophilized from PBS, pH 7.2.
 
Purity:≥90-95% (SDS-PAGE)
 
Purity Detail:His-tag-affinity purified.
 
Endotoxin Content:<0.00015EU/µg protein (LAL test; Charles River).
 
Biological Activity:Inhibits binding of LPS to CD14. Up to 5µg/ml mouse CD14 inhibit binding of FITC-LPS (0.5µg/ml) to mouse CD14+ CHO transfectants (FACS).
 
Reconstitution:Reconstitute with 40µl sterile, ultra-pure distilled water. This solution can then be diluted with PBS or other buffers.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:The reconstituted protein should be used immediately or stored in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% BSA).
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 ist the 50kDa protein.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Absence of TRIF signaling in lipopolysaccharide-stimulated murine mast cells: S. Keck, et al.; J. Immunol. 186, 5478 (2011), Abstract;
Monocytes can phagocytose Gram-negative bacteria by a CD14-dependent mechanism: U. Grunwald, et al.; J. Immunol. 157, 4119 (1996), Abstract;

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