Product Details
| MW: | ~52kDa (homodimer) |
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| Source: | Produced in E. coli. Active human granzyme A. |
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| EC: | 3.4.21.78 |
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| UniProt ID: | P12544 |
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| Concentration: | 0.2mg/ml |
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| Formulation: | Liquid. In 0.05M MES, pH 6.0, containing 10% glycerol and ~0.52M sodium chloride. Contains no preservatives. |
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| Purity: | ≥98% (SDS-PAGE) |
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| Specific Activity: | ~110U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol Z-Lys-SBzl per min. at 25°C in 0.05M TRIS, pH 8.0, containing 0.15M NaCl, 0.01% Triton X-100 and 0.3mM DTNB. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -80°C. Do not dilute to more than 0.2mg/ml. Do not store dilute solutions (1-1000ng/ml) for more than 24 hours. |
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| Handling: | For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Approaches to design non-covalent inhibitors for human granzyme B (hGrB): M. Kim, et al.; Org. Biomol. Chem.
12, 8952 (2014),
Abstract;
