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Nampt (Visfatin/PBEF) (human) Intracellular ELISA Kit

AG-45A-0006EK-KI01 96 wells Inquire for pricing
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The Nampt (Visfatin/PBEF) (human) Intracellular ELISA Kit is a complete assay for the quantitative determination of human Nampt (visfatin/PBEF) levels. This kit is to be used with cell lysates from human origin.

Product Details

Alternative Name:Nicotinamide Phosphoribosyltransferase, Pre-B-Cell Colony Enhancing Factor 1
Sensitivity:30pg/ml (range 0.25 – 16 ng Nampt/ml)
Application Notes:For the quantitative determination of human Nampt (visfatin/PBEF) in cell lysates.
Wavelength:450 nm
Species reactivity:Human
Specificity:Has been shown to specifically detect the physiologically relevant Nampt/visfatin dimer in human serum samples.
Crossreactivity:Does not cross-react with human adiponectin, human resistin, human RELM-β, human leptin, human GPX3, human ANGPTL4, human FABP4, human ANGPTL6, human PAI1. Shows 5% cross-reactivity with mouse Nampt and 15% cross reactivity with rat Nampt.
Shipping:Blue Ice
Long Term Storage:+4°C
Technical Info/Product Notes:Cited samples:
Cytokine ELISAs Cited Samples
UniProt ID:P43490
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Aerobic and resistance exercise training reverses age-dependent decline in NAD+ salvage capacity in human skeletal muscle: R.M. de Guia, et al.; Physiol. Rep. 7, e14139 (2019), Application(s): ELISA using human plasma, Abstract; Full Text
NADP+ is an endogenous PARP inhibitor in DNA damage response and tumor suppression: C. Bian, et al.; Nat. Commun. 10, 693 (2019), Application(s): ELISA using cell lysates, Abstract; Full Text
Ethnic differences in the role of adipocytokines linking abdominal adiposity and insulin sensitivity among asians: E. Parvaresh Rizi, et al.; J. Clin. Endocrinol. Metab. 100, 4249 (2015), Application(s): ELISA using human plasma, Abstract;
Visfatin in obese children and adolescents and its association with insulin resistance and metabolic syndrome: M. Nourbakhsh, et al.; Scand. J. Clin. Lab. Invest. 75, 183 (2015), Application(s): ELISA using human serum, Abstract;
Diurnal rhythm of circulating nicotinamide phosphoribosyltransferase (Nampt/visfatin/PBEF): impact of sleep loss and relation to glucose metabolism: C. Benedict, et al.; J. Clin. Endocrinol. Metab. 97, E218 (2012), Application(s): ELISA using human serum, Abstract;
Correlation of circulating full-length visfatin (PBEF/Nampt) with metabolic parameters in subjects with and without diabetes: a cross-sectional study: R. Retnakaran, et al.; Clin. Endocrinol. (Oxf) 69, 885 (2008), Abstract;
Serum levels of the adipokine visfatin are increased in pre-eclampsia: M. Fasshauer, et al.; Clin. Endocrinol. (Oxf) 69, 69 (2008), Abstract;
Molecular characteristics of serum visfatin and differential detection by immunoassays: A. Körner, et al.; J. Clin. Endocrinol. Metab. 92, 4783 (2007), Abstract; Full Text
Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway: T. Yonezawa, et al.; FEBS Lett. 580, 6635 (2006), Abstract;

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